Document Detail

Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts.
MedLine Citation:
PMID:  19250807     Owner:  NLM     Status:  MEDLINE    
This work investigated the biological influence of water-soluble multiwalled carbon nanotubes (wsMWCNTs) on fibroblast cell growth as a function of concentration control in an aqueous solution. The wsMWCNTs were prepared by an optimal procedure of ultrasonication/concentrated acids oxidation. The concentration of wsMWCNT in the solution was quantified by an established calibration line. A stable concentration of 0.3mg/ml was obtained in the surfactant-free water. The physicochemical properties of wsMWCNTs were characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), UV/VIS/NIR spectroscopy, and dynamic light scattering (DLS). Cell proliferation and the cell cycle were examined by MTS assay, flow cytometry and TEM respectively. Experimental results showed that the oxidation degree was a key factor that determined the concentration and stability of wsMWCNTs in the aqueous solution. The wsMWCNTs were able to enter into the cells and mainly accumulated in the cytoplasm. The wsMWCNTs-induced variations in cell proliferation and the cell cycle were concentration dependent. Cells cultivated with wsMWCNTs of 0.3mg/ml underwent a dramatic apoptosis. The proliferation was clearly suppressed when the cells were cultivated with wsMWCNTs of 0.03 mg/ml. There were no obvious influences on cell proliferation and the cell cycle when the concentration of wsMWNTs decreased to 0.01 mg/ml.
J Meng; M Yang; L Song; H Kong; C Y Wang; R Wang; C Wang; S S Xie; H Y Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-07
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  71     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-04-20     Completed Date:  2009-06-09     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  148-53     Citation Subset:  IM    
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
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MeSH Terms
3T3-L1 Cells
Apoptosis / drug effects
Cell Cycle / drug effects
Cell Proliferation / drug effects
Fibroblasts / cytology,  drug effects*,  ultrastructure
Flow Cytometry
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanotubes, Carbon / adverse effects*,  chemistry*
Reg. No./Substance:
0/Nanotubes, Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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