| Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts. | |
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MedLine Citation:
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PMID: 19250807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work investigated the biological influence of water-soluble multiwalled carbon nanotubes (wsMWCNTs) on fibroblast cell growth as a function of concentration control in an aqueous solution. The wsMWCNTs were prepared by an optimal procedure of ultrasonication/concentrated acids oxidation. The concentration of wsMWCNT in the solution was quantified by an established calibration line. A stable concentration of 0.3mg/ml was obtained in the surfactant-free water. The physicochemical properties of wsMWCNTs were characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), UV/VIS/NIR spectroscopy, and dynamic light scattering (DLS). Cell proliferation and the cell cycle were examined by MTS assay, flow cytometry and TEM respectively. Experimental results showed that the oxidation degree was a key factor that determined the concentration and stability of wsMWCNTs in the aqueous solution. The wsMWCNTs were able to enter into the cells and mainly accumulated in the cytoplasm. The wsMWCNTs-induced variations in cell proliferation and the cell cycle were concentration dependent. Cells cultivated with wsMWCNTs of 0.3mg/ml underwent a dramatic apoptosis. The proliferation was clearly suppressed when the cells were cultivated with wsMWCNTs of 0.03 mg/ml. There were no obvious influences on cell proliferation and the cell cycle when the concentration of wsMWNTs decreased to 0.01 mg/ml. |
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Authors:
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J Meng; M Yang; L Song; H Kong; C Y Wang; R Wang; C Wang; S S Xie; H Y Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-07 |
Journal Detail:
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Title: Colloids and surfaces. B, Biointerfaces Volume: 71 ISSN: 1873-4367 ISO Abbreviation: Colloids Surf B Biointerfaces Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-06-09 Revised Date: 2009-10-16 |
Medline Journal Info:
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Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: Netherlands |
Other Details:
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Languages: eng Pagination: 148-53 Citation Subset: IM |
Affiliation:
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Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Animals Apoptosis / drug effects Cell Cycle / drug effects Cell Proliferation / drug effects Fibroblasts / cytology, drug effects*, ultrastructure Flow Cytometry Mice Microscopy, Electron, Scanning Microscopy, Electron, Transmission Nanotubes, Carbon / adverse effects*, chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Nanotubes, Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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