Document Detail


Concentration of Giardia lamblia cysts, Legionella pneumophila, Clostridium perfringens, human enteric viruses, and coliphages from large volumes of drinking water, using a single filtration.
MedLine Citation:
PMID:  2555036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poliovirus, coliphages, Giardia lamblia cysts, Clostridium perfringens spores, and Legionella pneumophila were concentrated simultaneously in a single pass by sequential filtration of large volumes of drinking water through 3- and 1-micron wound electronegative fiberglass cartridge filters (25.4 cm). Filtration was performed under acidic conditions (pH 3.5) in the presence of 0.001 M aluminum chloride to enhance adsorption. Elution of all the microorganisms entrapped or adsorbed to the filters was obtained by a slow backwash elution with a 1.5% beef extract solution, pH 9.75, containing 0.5% Tween 80. Tween 80 was shown to enhance recovery of the bacteriophages, bacteria, and parasites. Giardia cysts were efficiently eluted (71%) and could be reconcentrated by low-speed centrifugation and purified by sucrose density gradient flotation at a final recovery of 52%. Legionella pneumophila cells were eluted at 64% and were further concentrated by low-speed centrifugation at an overall recovery of 55%. C. perfringens spores and coliphages were eluted at efficiencies of 82 and 86%, respectively, and reconcentrated with minimal loss by a detergent - protein flotation method. Poliovirus was eluted at 93% and reconcentrated at 78% efficiency by organic flocculation.
Authors:
P Payment; A Bérubé; D Perreault; R Armon; M Trudel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  35     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-12-26     Completed Date:  1989-12-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  932-5     Citation Subset:  IM    
Affiliation:
Centre de recherche en virologie, Université du Québec, Laval, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Clostridium perfringens / isolation & purification
Coliphages / isolation & purification
Enterovirus / isolation & purification
Filtration*
Giardia / isolation & purification
Legionella / isolation & purification
Water Microbiology*
Water Pollution*
Water Supply*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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