Document Detail

Concanavalin A surface receptors and cytoplasmic actin in cell adhesion.
MedLine Citation:
PMID:  396917     Owner:  NLM     Status:  MEDLINE    
A double immunofluorescence staining technique to locate concanavalin A (Con A) surface receptors and cytoplasmic actin in the same cell was applied to monolayer cultures of rat foetal fibroblasts during cell detachment induced by trypsin and during cell attachment to glass substratum. Con A receptors were demonstrated by fluorescein-isothiocyanate-labelled Con A (FITC-Con A) and actin by specific anti-actin antibody (AAA) traced with rhodamine-labelled goat anti-human globulin (R-AHG). Untreated, control cells had an elongated shape, Con A receptors restricted to cell margins and prominent actin filaments. After 2 min treatment with 0.001% trypsin the cells became angular with Con A receptors in clusters and actin in a diffuse or aggreagate staining pattern. Progressive cell rounding followed and this was accompanied by the development of long, thin, arborized cell processes, studded with Con A receptors and containing fine actin filaments. Complete cell rounding preceded cell detachment. The sites of detached cells were marked by fine aggregates containing Con A receptors and actin. In cells attaching to a glass substratum, actin was present in a diffusely stained or aggregate pattern in round cells, in filaments restricted to cell margins in partially spread cells and in numerous filaments in fully spread cells. Con A receptors were present in clusters in round cells, in clusters or caps in partially spread cells and in cell margins in fully spread cells. Binding of FITC-Con A to partially spread cells resulted in dissolution of the few, newly formed, actin filaments. We believe our observations are consistent with the idea that actin filaments, formed during cell attachment, contribute towards the maintenance of cell adhesion by helping in the preservation of cell shape and by anchorage of Con A receptors at points of cell attachment to the substratum.
B H Toh; V B Randell; S S Prime
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Australian journal of experimental biology and medical science     Volume:  57     ISSN:  0004-945X     ISO Abbreviation:  Aust J Exp Biol Med Sci     Publication Date:  1979 Aug 
Date Detail:
Created Date:  1980-06-25     Completed Date:  1980-06-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0416662     Medline TA:  Aust J Exp Biol Med Sci     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  429-41     Citation Subset:  IM    
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MeSH Terms
Actins / isolation & purification*
Cell Adhesion* / drug effects
Culture Techniques
Cytoplasm / analysis
Embryo, Mammalian
Fluorescent Antibody Technique
Receptors, Concanavalin A / isolation & purification*
Trypsin / pharmacology
Reg. No./Substance:
0/Actins; 0/Receptors, Concanavalin A; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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