Document Detail

Computer image analysis of two-dimensional crystals of beef heart NADH: ubiquinone oxidoreductase fragments. II. Comparison of frozen hydrated and negatively stained specimens.
MedLine Citation:
PMID:  2467418     Owner:  NLM     Status:  MEDLINE    
Two-dimensional crystals of bovine NADH: ubiquinone oxidoreductase fragments were studied by cryo-electron microscopy and computer image analysis at two focus settings. The lattice parameters of the crystals were determined as a = 14.5 +/- 0.2 nm, b = 14.8 +/- 0.3 nm with gamma = 87.8 degrees +/- 0.2 degrees. Translation and rotation alignment resulted in a final resolution of 1.38 (close-to-focus) or 1.9 nm (1.3 microns underfocus). When only translational alignment was employed 1.6 nm resolution resulted (close-to-focus case). The application of a further correction procedure surprisingly lowered the achieved resolution to 2.2 nm. A combination of the projected structures for the two focus settings in Fourier space resulted in a noise-free image displaying enhanced image contrast for reciprocal spacings from 1/5.0 to 1/1.4 nm-1. The structure in amorphous ice comprises four separate quasi-identical motifs, each resembling a distorted triangle. Two symmetry-related (p2) motifs reveal a pore-like feature. In negative stain the structure has p4mm symmetry. The absence of in-plane symmetry elements (i.e. twofold rotation and screw axes) and other differences might be accounted for (at least in part) if the crystal studied was slightly tilted.
J Brink; F P Booy; E F Van Bruggen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ultramicroscopy     Volume:  27     ISSN:  0304-3991     ISO Abbreviation:  Ultramicroscopy     Publication Date:    1989 Jan-Feb
Date Detail:
Created Date:  1989-05-05     Completed Date:  1989-05-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7513702     Medline TA:  Ultramicroscopy     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  91-9     Citation Subset:  IM    
Biochemisch Laboratorium, Rijksuniversiteit Groningen, The Netherlands.
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MeSH Terms
Image Processing, Computer-Assisted*
Microscopy, Electron / methods*
Myocardium / enzymology
NAD(P)H Dehydrogenase (Quinone)
Quinone Reductases / analysis*
Staining and Labeling*
Reg. No./Substance:
EC Dehydrogenase (Quinone); EC 1.6.99.-/Quinone Reductases

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