Document Detail

Computer experiments to determine whether over- or under-counting necessarily affects the determination of difference in cell number between experimental groups.
MedLine Citation:
PMID:  11248344     Owner:  NLM     Status:  MEDLINE    
Computer cell counting experiments were performed in order to examine the consequences of over- or under-counting. The three-dimensional reaggregate culture laboratory environment for cell counting was used as a model for computer simulation. The laboratory environment for aggregate and cell sizes, numbers and spatial placement in gelatin blocks was mimicked in the computer setup. However, in the computer, cell counting was set to be either ideally unbiased, or deliberately biased in regard to over- or under-counting so as to compare eventual results when using the various cell counting methods. It was found that there was no effect of the cell counting methods used in determining whether there was a significant difference in cell number between two experimental groups. In addition, it was found that under the conditions of these simulations, the optical dissector method behaved similarly, on the average, as the ideal method of counting cell centers and in both of those cases, the average ratio between actual cell number in a flask and estimated number was close to 1.00.
B Heller; F Schweingruber; D Guvenc; A Heller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  106     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-15     Completed Date:  2001-06-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-9     Citation Subset:  IM    
Department of Applied Mathematics, Illinois Institute of Technology, Chicago, IL, USA.
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MeSH Terms
Cell Count / instrumentation,  methods
Computer Simulation*
Corpus Striatum / cytology
Image Processing, Computer-Assisted / methods*
Mesencephalon / cytology
Models, Biological*
Grant Support

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