| Computer experiments to determine whether over- or under-counting necessarily affects the determination of difference in cell number between experimental groups. | |
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MedLine Citation:
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PMID: 11248344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Computer cell counting experiments were performed in order to examine the consequences of over- or under-counting. The three-dimensional reaggregate culture laboratory environment for cell counting was used as a model for computer simulation. The laboratory environment for aggregate and cell sizes, numbers and spatial placement in gelatin blocks was mimicked in the computer setup. However, in the computer, cell counting was set to be either ideally unbiased, or deliberately biased in regard to over- or under-counting so as to compare eventual results when using the various cell counting methods. It was found that there was no effect of the cell counting methods used in determining whether there was a significant difference in cell number between two experimental groups. In addition, it was found that under the conditions of these simulations, the optical dissector method behaved similarly, on the average, as the ideal method of counting cell centers and in both of those cases, the average ratio between actual cell number in a flask and estimated number was close to 1.00. |
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Authors:
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B Heller; F Schweingruber; D Guvenc; A Heller |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 106 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-03-15 Completed Date: 2001-06-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 91-9 Citation Subset: IM |
Affiliation:
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Department of Applied Mathematics, Illinois Institute of Technology, Chicago, IL, USA. effe@midway.uchicago.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Count / instrumentation, methods Computer Simulation* Corpus Striatum / cytology Image Processing, Computer-Assisted / methods* Mesencephalon / cytology Models, Biological* |
| Grant Support | |
ID/Acronym/Agency:
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DA-0964/DA/NIDA NIH HHS; MH-28942/MH/NIMH NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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