Document Detail

Computer-controlled double-beam scanning microspectrophotometry for rapid microscopic image reconstructions.
MedLine Citation:
PMID:  95455     Owner:  NLM     Status:  MEDLINE    
A method for automated collection of various specific data from an entire microscopical preparation and their quantitative evaluation is described. Its application to the study of neuronal connections is discussed in some detail. Brain sections are scanned using a computer-controlled microscope for reflectance, fluorescences or absorbance signals. Two illuminating beams are used, one of them being amplitude modulated. By means of a synchronous detection the two signals are recorded simultaneously: for example, in an autoradiograph, the reflectance (measuring the density of the silver grains in emulsion) and the absorbance (allowing to localize the underlying counterstained cells). The data are stored in a computer. Various off-line processing schemes allow the reconstruction of the data with respect to the corresponding spatial coordinates. Thus pseudo-three-dimensional, analogue or digital, graphic displays may be obtained in which the patterns of neuronal connections can be recognized and interpreted. A method for the detection of weakly labelled nerve fibres based on digital filtering is presented. The whole processing for a frontal section of the mouse brain (7 X 10 mm area) takes less than 1 h. In addition to the evaluation of microscopically labelled material (grains of autoradiographs, horseradish peroxidase, nucleic acids) the technique described has been successfully used for the study of naturally fluorescent intracellular components in living tissue cultures.
P Kucera; Y de Ribaupierre; F de Ribaupierre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microscopy     Volume:  116     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1981-03-27     Completed Date:  1981-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  173-84     Citation Subset:  IM    
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MeSH Terms
Brain / cytology
Chick Embryo
Microscopy / methods*
Neural Pathways*
Neurons / cytology
Staining and Labeling

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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