| Computer-controlled double-beam scanning microspectrophotometry for rapid microscopic image reconstructions. | |
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MedLine Citation:
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PMID: 95455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method for automated collection of various specific data from an entire microscopical preparation and their quantitative evaluation is described. Its application to the study of neuronal connections is discussed in some detail. Brain sections are scanned using a computer-controlled microscope for reflectance, fluorescences or absorbance signals. Two illuminating beams are used, one of them being amplitude modulated. By means of a synchronous detection the two signals are recorded simultaneously: for example, in an autoradiograph, the reflectance (measuring the density of the silver grains in emulsion) and the absorbance (allowing to localize the underlying counterstained cells). The data are stored in a computer. Various off-line processing schemes allow the reconstruction of the data with respect to the corresponding spatial coordinates. Thus pseudo-three-dimensional, analogue or digital, graphic displays may be obtained in which the patterns of neuronal connections can be recognized and interpreted. A method for the detection of weakly labelled nerve fibres based on digital filtering is presented. The whole processing for a frontal section of the mouse brain (7 X 10 mm area) takes less than 1 h. In addition to the evaluation of microscopically labelled material (grains of autoradiographs, horseradish peroxidase, nucleic acids) the technique described has been successfully used for the study of naturally fluorescent intracellular components in living tissue cultures. |
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Authors:
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P Kucera; Y de Ribaupierre; F de Ribaupierre |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of microscopy Volume: 116 ISSN: 0022-2720 ISO Abbreviation: J Microsc Publication Date: 1979 Jul |
Date Detail:
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Created Date: 1981-03-27 Completed Date: 1981-03-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0204522 Medline TA: J Microsc Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 173-84 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Brain / cytology Chick Embryo Computers Mice Microscopy / methods* Neural Pathways* Neurons / cytology Spectrophotometry* Staining and Labeling |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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