Document Detail

Computer-averaged views of the 70 S monosome from Escherichia coli.
MedLine Citation:
PMID:  3519978     Owner:  NLM     Status:  MEDLINE    
The prokaryotic (70 S) monosome, composed of a roughly hemispherical 50 S large subunit and an elongate 30 S small subunit, appears in the electron micrograph in only a few common views representing the small number of preferred orientations assumed by the particle. Two of these, termed O and L views, have previously been characterized as the overlap and non-overlap projections; a third view, which we term the R view, represents the other endpoint of a rotational continuum with the overlap or O view. Tilt studies enabled us to calibrate this range as spanning approximately 50 degrees. The disjunct set of L views was averaged, and the reproducible resolution was determined to be 1/3.5 nm-1. The combined sets of O and R views were analyzed by correspondence analysis, and a continuous "rotation series" of subaverages was obtained. Interpretation of the views in the light of what is known about the morphologies of the individual subunits allows a general picture of the mutual fit of the subunits in the monosome to be conceived.
A Verschoor; J Frank; T Wagenknecht; M Boublik
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular biology     Volume:  187     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-06-30     Completed Date:  1986-06-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  581-90     Citation Subset:  IM    
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MeSH Terms
Escherichia coli / ultrastructure*
Macromolecular Substances
Microscopy, Electron
Ribosomes / analysis*
Grant Support
Reg. No./Substance:
0/Macromolecular Substances

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