Document Detail

Computer-assisted microscope characterization of BCNU-induced modifications in the collective behavior of 12 human brain cancer cell lines.
MedLine Citation:
PMID:  8740586     Owner:  NLM     Status:  MEDLINE    
The aim of our study is to characterize the disturbance induced by repeated BCNU treatments in 12 human brain tumor cell lines in terms of their collective behavior. This collective behavior was characterized by means of the Delaunay triangulation and Voronoi mathematical paving techniques combined with the computer-assisted microscope analysis of Feulgen-stained nuclei. This methodology enabled growth to be characterized in terms of cell colony size and density. In addition to this colony pattern characterization, the DNA ploidy level was assessed by means of DNA histogram typing. The cell proliferation level was also determined. Ten astrocytic and two medulloblastoma cell lines treated weekly with BCNU were analyzed. Study of the cell colony architecture and cell proliferation revealed specific BCNU-induced modifications in connection with the origins of the cell lines, i.e. astrocytoma (AST), glioblastoma (GBM), or medulloblastoma (MED). The BCNU-induced effect on GBM (the more malignant of the cell lines) was very different in that proliferation was weakened, but the cell colony density increased after a latency phase. The decrease in cell colony density and cell proliferation of MED seems to indicate that they are more sensitive to BCNU than GBM, but relatively tolerant of this type of chemotherapy in comparison with AST.
I Camby; I Salmon; A Danguy; J L Pasteels; R Kiss
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  28     ISSN:  0167-594X     ISO Abbreviation:  J. Neurooncol.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-10-31     Completed Date:  1996-10-31     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Laboratoire d'histologie, Faculté de Médecine, Université Libre de Bruxelles.
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MeSH Terms
Brain Neoplasms*
Carcinogens / pharmacology*
Carmustine / pharmacology*
Cell Count
Cell Death / drug effects,  genetics
Cell Division / drug effects,  genetics
Cell Survival / drug effects,  genetics
Coloring Agents
DNA, Neoplasm / analysis,  drug effects
Image Processing, Computer-Assisted
Rosaniline Dyes*
Tumor Cells, Cultured / cytology,  drug effects
Reg. No./Substance:
0/Carcinogens; 0/Coloring Agents; 0/DNA, Neoplasm; 0/Feulgen stain; 0/Rosaniline Dyes; 154-93-8/Carmustine

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