Document Detail

Computer-assisted immunocytochemical determination of breast cancer steroid receptors on cytological smears of excised surgical specimens compared with frozen sections.
MedLine Citation:
PMID:  10589046     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Due to the widespread use of fine needle aspirate biopsy the practice of determining estrogen (ER) and progesterone (PR) receptors in breast carcinoma from cytological smears (CS) is becoming very common. The aim of this study was to determine concordance between ER and PR assessed by immunocytochemical assay (ICA) on CS and FS both evaluated by image analysis since we have found no data in literature on this. METHODS: 104 breast carcinoma cases were selected. For all cases ER and PR determination was performed on CS, obtained by light scraping of the freshly cut surface of the excised surgical tumors at the time of frozen section diagnosis, and FS using the same monoclonal antibodies. Computer-assisted image analysis was performed in all cases using CAS 200. Results were expressed as percent positive area of neoplastic nuclei compared with total nuclear area of the examined neoplastic cells. RESULTS: Good correlation was demonstrated between percent positive nuclear neoplastic area by ER-ICA on CS and FS (r = 0.759; P < 0.0001). Concordance of results was 90.19% (P < 0.001). Good correlation was also demonstrated between percent positive nuclear neoplastic area by PR-ICA in CS and FS (r = 0.889; P < 0. 0001). Concordance of results was 97.02% (P < 0.0001). CONCLUSIONS: Our data suggest that ICA on CS with automated image analysis is efficient in evaluating ER and PR content in human breast cancer, especially when CS is the only method pathologists have to evaluate receptor status e.g. in advanced breast cancer cases when neoadjuvant therapy is necessary before surgery or when surgery is impossible.
A Cavaliere; A Sidoni; I Ferri; R Vitali; E Bucciarelli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry     Volume:  38     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-15     Completed Date:  2000-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-5     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Wiley-Liss, Inc.
Institute of Pathological Anatomy and Histology, Division of Cancer Research, Perugia University, Perugia, Italy.
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MeSH Terms
Aged, 80 and over
Breast Neoplasms / metabolism*
Breast Neoplasms, Male / metabolism
Carcinoma, Ductal, Breast / metabolism
Cytological Techniques
Frozen Sections
Image Processing, Computer-Assisted
Immunohistochemistry / methods*
Middle Aged
Receptors, Estrogen / metabolism*
Receptors, Progesterone / metabolism*
Reg. No./Substance:
0/Receptors, Estrogen; 0/Receptors, Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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