Document Detail


Computer-assisted imaging cytometry of nuclear chromatin reveals bone tumor virus infection and neoplastic transformation of adherent osteoblast-like cells.
MedLine Citation:
PMID:  2554899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Established osteoblast-like (OB) cells infected with the bone tumor-inducing C-type retrovirus OA MuLV remained nontumorigenic over 104 cell culture passages. DNA histograms revealed a new cell population with a stem line peak at 5c. A second OA MuLV-infected OB cell line underwent neoplastic transformation with increasing passage level. These cells showed diffuse aneuploidy. Stepwise linear discriminant analysis of the chromatin structure of control, OA MuLV-infected, and FBR osteosarcoma virus-transformed cell lines resulted in various levels of discrimination ranging between 79.6% for control cells versus nontumorigenic OA MuLV-infected cells, and 96.6% for nontumorigenic OA MuLV-infected cells versus FBR osteosarcoma virus-transformed cells. OA MuLV-infected tumorigenic cells and FBR osteosarcoma virus-transformed cells were discriminated at a 93.6% level.
Authors:
J Schmidt; M Aubele; U Jütting; K Rodenacker; A Luz; V Erfle; G Burger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  164     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-12-11     Completed Date:  1989-12-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  728-35     Citation Subset:  IM    
Affiliation:
GSF-Abteilung für Molekulare Zellpathologie, Institut für Strahlenschutz, Neuherberg, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Cell Line
Cell Nucleus / microbiology*,  ultrastructure
Cell Transformation, Neoplastic*
Chromatin / microbiology*,  ultrastructure
Clone Cells
Computers
Flow Cytometry / methods
Leukemia Virus, Murine / pathogenicity,  ultrastructure*
Leukemia, Experimental / pathology*
Mice
Osteoblasts / microbiology*,  ultrastructure
Chemical
Reg. No./Substance:
0/Chromatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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