Document Detail


Computed tomography monitoring of radiation-induced lung fibrosis in mice.
MedLine Citation:
PMID:  15377939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE AND OBJECTIVES: To identify characteristics of lung fibrosis in a mouse model after radiotherapy (RT) using thin-section computed tomography (CT), histology and clinical parameters. MATERIALS AND METHOD: Using a multislice CT-scanner, follow-up chest CT scans of 10 out of 72 included mice (C57BL/6J, 36 control mice, 36 mice (20Gy)) were performed every 2 weeks until week 26 after RT. Hounsfield units (HU) and cardiothoracic ratio (CTR) were measured, and a multireader analysis on characteristic lung changes was performed and correlated with histology and clinical parameters. RESULTS: From weeks 4 to 8 after RT changes in histology (leukocyte count, extraalveolar edema, P < 0.01) and from week 12 changes in CT were detected (increase in HU, intralobular opacity and fibrotic strandings, P < 0.05). From week 14 clinical manifestations occurred (loss of weight, mobility, breathing, increased mortality, P < 0.01). CTR showed no significant changes. Three readers showed excellent interobserver agreement (kappa >0.84). CONCLUSION: Thin-section CT in a mouse model is capable of detecting the development of lung fibrosis after RT prior to the onset of clinical deterioration.
Authors:
Christian Plathow; Minglun Li; Ping Gong; Heike Zieher; Fabian Kiessling; Peter Peschke; Hans-Ulrich Kauczor; Amir Abdollahi; Peter E Huber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative radiology     Volume:  39     ISSN:  0020-9996     ISO Abbreviation:  Invest Radiol     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-20     Completed Date:  2005-02-08     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0045377     Medline TA:  Invest Radiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  600-9     Citation Subset:  IM    
Affiliation:
Department of Radiology, Deutsches Krebsforschungszentrum Heidelberg, Germany. c.plathow@dkfz.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Edema
Female
Histological Techniques
Leukocyte Count
Mice
Mice, Inbred C57BL
Models, Animal
Monitoring, Physiologic
Pulmonary Fibrosis / physiopathology*,  radiography*
Radiation Injuries, Experimental / physiopathology*
Thorax / radiation effects
Tomography, X-Ray Computed*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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