Document Detail


Computational analyses of carbon monoxide (CO) rebreathing methods for estimating hemoglobin mass in humans.
MedLine Citation:
PMID:  21948194     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Measurement of hemoglobin mass (MHb) is used to quantify alterations in oxygen delivery during exercise training or acclimatization to altitude. Uptake of carbon monoxide (CO) by hemoglobin is the basis of the common non-radioactive methods to determine MHb in humans. This study used a validated mathematical model to simulate CO uptake during rebreathing protocols and to determine sources of errors in estimation of MHb. Our previously published model (Erupaka et al. 2010) was validated using experimentally measured carboxyhemoglobin levels (%HbCO) from arterial, capillary and venous blood sites of human subjects (Garvican et al. 2010) during CO rebreathing protocols. This model was then used to simulate various CO rebreathing protocols in 24 human subjects with known MHb. Using variables generated by the model, MHb was estimated on the basis of assumptions typically made for calculating volume of CO bound to myoglobin, volume of CO exhaled, and the volume of CO in the rebreathing system. It was found that inaccurate estimation of volume of CO bound to myoglobin was the major source of error in determination of MHb. Additionally, the size of the error was found to depend on the site of blood sampling because of differences in %HbCO. Regression equations are developed to improve the estimation of volume of CO bound to myoglobin, and a new protocol that is less dependent on the site of blood sampling is proposed.
Authors:
Kinnera E Chada; Eugene N Bruce
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-23
Journal Detail:
Title:  Experimental physiology     Volume:  -     ISSN:  1469-445X     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of Kentucky.
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