Document Detail


Computational Optogenetics: A Novel Continuum Framework for the Photoelectrochemistry of Living Systems.
MedLine Citation:
PMID:  22773861     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Electrical stimulation is currently the gold standard treatment for heart rhythm disorders. However, electrical pacing is associated with technical limitations and unavoidable potential complications. Recent developments now enable the stimulation of mammalian cells with light using a novel technology known as optogenetics. The optical stimulation of genetically engineered cells has significantly changed our understanding of electrically excitable tissues, paving the way towards controlling heart rhythm disorders by means of photostimulation. Controlling these disorders, in turn, restores coordinated force generation to avoid sudden cardiac death. Here, we report a novel continuum framework for the photoelectrochemistry of living systems that allows us to decipher the mechanisms by which this technology regulates the electrical and mechanical function of the heart. Using a modular multiscale approach, we introduce a non-selective cation channel, channelrhodopsin-2, into a conventional cardiac muscle cell model via an additional photocurrent governed by a light-sensitive gating variable. Upon optical stimulation, this channel opens and allows sodium ions to enter the cell, inducing electrical activation. In side-by-side comparisons with conventional heart muscle cells, we show that photostimulation directly increases the sodium concentration, which indirectly decreases the potassium concentration in the cell, while all other characteristics of the cell remain virtually unchanged. We integrate our model cells into a continuum model for excitable tissue using a nonlinear parabolic second order partial differential equation, which we discretize in time using finite differences and in space using finite elements. To illustrate the potential of this computational model, we virtually inject our photosensitive cells into different locations of a human heart, and explore its activation sequences upon photostimulation. Our computational optogenetics tool box allows us to virtually probe landscapes of process parameters, and to identify optimal photostimulation sequences with the goal to pace human hearts with light and, ultimately, to restore mechanical function.
Authors:
Jonathan Wong; Oscar J Abilez; Ellen Kuhl
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of the mechanics and physics of solids     Volume:  60     ISSN:  0022-5096     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9890138     Medline TA:  J Mech Phys Solids     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1158-1178     Citation Subset:  -    
Affiliation:
Department of Mechanical Engineering, Stanford University, Stanford, CA 94305, USA, jonjwong@stanford.edu.
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