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Computational Analyses of Intravascular Tracer Washout Reveal Altered Capillary-Level Flow Distributions in Obese Zucker Rats.
MedLine Citation:
PMID:  21788350     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Intravascular tracer washout data obtained from gastrocnemius muscle of lean Zucker rats (LZR) and obese Zucker rats (OZR) is analyzed to investigate flow distributions in OZR, a model of non-atherosclerotic peripheral vascular disease. A computer model used to simulate the network washout curves is developed based on experimentally-observed relative dispersions in large vessels and asymmetrical flow distributions at bifurcations in dichotomous microvascular networks. The model results of simulations are compared to experimental washout data of (125)I-albumin, an intravascular tracer, to uncover flow distributions on the arterial-network and capillary levels. The lean and obese Zucker rats demonstrate distinct capillary-level flow distributions, with higher dispersion and significantly more low-flow capillaries in the OZR than in the LZR. Targeted pharmacological treatments against identified sites of vascular dysfunction in OZR (adrenoreceptor blockade with phentolamine, antioxidant treatment with TEMPOL and thromboxane receptor antagonism with SQ-29548) are shown to improve the capillary-level flow distributions in treated OZR toward distributions determined in control LZR. Combination therapy with multiple pharmacological interventions resulted in a greater degree of recovery. This study demonstrates that the enhanced perfusion heterogeneity at arteriole bifurcations is a potential mechanism underlying perfusion-demand mismatching in OZR, and suggests that amelioration of this dysfunction must involve a multi-faceted interventional approach.
Authors:
Fan Wu; Daniel A Beard; Jefferson C Frisbee
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-25
Journal Detail:
Title:  The Journal of physiology     Volume:  -     ISSN:  1469-7793     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Medical College of Wisconsin;
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