Document Detail

Compromised energetics in the adenylate kinase AK1 gene knockout heart under metabolic stress.
MedLine Citation:
PMID:  11006295     Owner:  NLM     Status:  MEDLINE    
Rapid exchange of high energy carrying molecules between intracellular compartments is essential in sustaining cellular energetic homeostasis. Adenylate kinase (AK)-catalyzed transfer of adenine nucleotide beta- and gamma-phosphoryls has been implicated in intracellular energy communication and nucleotide metabolism. To demonstrate the significance of this reaction in cardiac energetics, phosphotransfer dynamics were determined by [(18)O]phosphoryl oxygen analysis using( 31)P NMR and mass spectrometry. In hearts with a null mutation of the AK1 gene, which encodes the major AK isoform, total AK activity and beta-phosphoryl transfer was reduced by 94% and 36%, respectively. This was associated with up-regulation of phosphoryl flux through remaining minor AK isoforms and the glycolytic phosphotransfer enzyme, 3-phosphoglycerate kinase. In the absence of metabolic stress, deletion of AK1 did not translate into gross abnormalities in nucleotide levels, gamma-ATP turnover rate or creatine kinase-catalyzed phosphotransfer. However, under hypoxia AK1-deficient hearts, compared with the wild type, had a blunted AK-catalyzed phosphotransfer response, lowered intracellular ATP levels, increased P(i)/ATP ratio, and suppressed generation of adenosine. Thus, although lack of AK1 phosphotransfer can be compensated in the absence of metabolic challenge, under hypoxia AK1-knockout hearts display compromised energetics and impaired cardioprotective signaling. This study, therefore, provides first direct evidence that AK1 is essential in maintaining myocardial energetic homeostasis, in particular under metabolic stress.
D Pucar; E Janssen; P P Dzeja; N Juranic; S Macura; B Wieringa; A Terzic
Related Documents :
24741075 - Somg-833, a novel, selective c-met inhibitor, blocks c-met dependent neoplastic effects...
8660605 - Measurement of the protein tyrosine kinase activity of c-src using time-resolved fluoro...
17158705 - How phosphotransferase system-related protein phosphorylation regulates carbohydrate me...
11483495 - X-ray structure of hpr kinase: a bacterial protein kinase with a p-loop nucleotide-bind...
12537555 - The repertoire of protein kinases encoded in the draft version of the human genome: aty...
3004485 - Kinetic properties of glycogen synthase from skeletal muscle after phosphorylation by g...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  275     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-25     Completed Date:  2001-01-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  41424-9     Citation Subset:  IM    
Division of Cardiovascular Diseases, Departments of Medicine, Molecular Pharmacology and Experimental Therapeutics, and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenosine Triphosphate / analysis
Adenylate Kinase / genetics,  physiology*
Energy Metabolism*
Mice, Knockout
Myocardium / metabolism*
Grant Support
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces ce...
Next Document:  Novel catalytic mechanism of nucleophilic substitution by asparagine residue involving cyanoalanine ...