Document Detail

Compression and crevasses in vitreous sections under different cutting conditions.
MedLine Citation:
PMID:  18445144     Owner:  NLM     Status:  MEDLINE    
Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.
H-M Han; B Zuber; J Dubochet
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  230     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-06-26     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  167-71     Citation Subset:  IM    
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MeSH Terms
Cryoelectron Microscopy / methods*
Cryoultramicrotomy / instrumentation*,  methods
Enterococcus faecalis / growth & development,  ultrastructure*
Frozen Sections
Grant Support
MC_U105184294//Medical Research Council

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