| Comprehensive proteomic analysis of host cell lipid rafts modified by HBV infection. | |
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MedLine Citation:
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PMID: 21982977 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Lipid rafts are cholesterol- and sphingolipid-rich membrane microdomains that have been shown to participate in the entry, assembly and budding of various viruses. However, their involvement in HBV replication remains poorly characterized. In a preliminary study, we observed that HBV release could be markedly impaired by methyl-β-cyclodextrin mediated depletion of cholesterol in lipid rafts, and that this effect could be reversed by replenishment of exogenous cholesterol, suggesting that lipid rafts play an important role in the HBV life cycle. To further understanding how HBV exploited host cell lipid rafts to benefit replication, comprehensive proteomic approaches were used to profile the proteome changes of host cell lipid rafts in response to HBV infection using 2DE-MS/MS, in combination with SILAC-based quantitative proteomics. Using these approaches, a total of 97 differentially expressed proteins were identified. Bioinformatics analysis suggested that multiple host cell pathways were involved in the HBV infection processes including signal transduction, metabolism, immune response, transport, vesicle trafficking, cell adhesion and cellular ion homeostasis. These data will provide valuable clues for further investigation of HBV pathogenesis. |
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Authors:
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Na Xie; Kai Huang; Tao Zhang; Yunlong Lei; Rui Liu; Kui Wang; Shengtao Zhou; Jingyi Li; Jinhua Wu; Hong Wu; Cao Deng; Edouard Collins Nice; Canhua Huang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-29 |
Journal Detail:
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Title: Journal of proteomics Volume: - ISSN: 1876-7737 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-10-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101475056 Medline TA: J Proteomics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
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The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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