| Comprehensive profiling of the human circulating endocannabinoid metabolome: clinical sampling and sample storage parameters. | |
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MedLine Citation:
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PMID: 18611105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Endogenous cannabinoid-receptor ligands (endocannabinoids) and over a dozen related metabolites now comprise the "endocannabinoid metabolome". The diverse (patho)physiological roles of endocannabinoids, the predictive/diagnostic utility of systemic endocannabinoid levels, and the growing interest in endocannabinoid-related pharmacotherapeutics mandate a valid clinical protocol for processing human blood that does not jeopardize profiling of the circulating endocannabinoid metabolome. METHODS: We systematically evaluated the potential effect of pre-analytical variables associated with phlebotomy and sample handling/work-up on the human-blood endocannabinoid metabolome as quantified by state-of-the-art liquid chromatography-mass spectrometry. RESULTS: Neither subject posture during phlebotomy nor moderate activity beforehand influenced the blood levels of the 15 endocannabinoid-system lipids quantified. Storage of fresh blood at 4 degrees C selectively enhanced ethanolamide concentrations artifactually without affecting monoglycerides and nonesterified fatty acids, such as arachidonic acid. In marked contrast, ethanolamides and monoglycerides remained stable through three plasma freeze/thaw cycles, whereas plasma arachidonic acid content increased, probably a reflection of ongoing metabolism. CONCLUSIONS: Class- and compound-selective pre-analytical influences on circulating human endocannabinoid levels necessitate immediate plasma preparation from fresh blood and prompt plasma apportioning and snap-freezing. Repeated plasma thawing and refreezing should be avoided. This protocol ensures sample integrity for evaluating the circulating endocannabinoid metabolome in the clinical setting. |
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Authors:
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JodiAnne T Wood; John S Williams; Lakshmipathi Pandarinathan; Amber Courville; Melissa R Keplinger; David R Janero; Paul Vouros; Alexandros Makriyannis; Carol J Lammi-Keefe |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 46 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-09-12 Completed Date: 2008-12-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 1289-95 Citation Subset: IM |
Affiliation:
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Center for Drug Discovery, Northeastern University, Boston, MA 02115, USA. j.wood@neu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Animals Blood Circulation* Blood Specimen Collection / methods* Cattle Endocannabinoids / blood*, isolation & purification, metabolism* Female Humans Motor Activity Posture Reference Values Sensitivity and Specificity Temperature |
| Grant Support | |
ID/Acronym/Agency:
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P01-DA9158/DA/NIDA NIH HHS; T32-DA7312/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Endocannabinoids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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