| Comprehensive evaluation of two HLA-B17 monoclonal antibodies for flow cytometry-based HLA-B57/B58 screening prior to abacavir prescription. | |
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MedLine Citation:
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PMID: 23280011 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Hypersensitivity reactions to the drug abacavir, used to treat HIV/AIDS patients, is associated with possession of HLA-B*57:01. We have carefully assessed two commercially available HLA-B57/B58 murine monoclonal antibodies [0196HA and BIH0243 (One Lambda Inc.)] in a simple flow cytometry-based assay. The evaluation involved tests on 228 reference and random samples covering 91% of all WHO recognized HLA-A, B and C specificities. These involved donors with six different HLA-B*57 alleles and included 19 examples of B*57:01. Both antibodies unambiguously detected B57, but there were small difference in their reactivity against B57-positive non-B*57:01 samples. Importantly, there was no reactivity against B57/B58-negative samples. The possible amino acid motifs involved in the reactivity of these antibodies with B57/B58 were delineated. Thus, HLA-B57/B58, normally present in <10% of patients, can be easily recognized using these two antibodies and further tested by a DNA-based typing method to identify B*57:01. |
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Authors:
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R Stevens; E Coates; J Street; E Cook; C Darke |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-26 |
Journal Detail:
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Title: International journal of immunogenetics Volume: - ISSN: 1744-313X ISO Abbreviation: Int. J. Immunogenet. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2013-1-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101232337 Medline TA: Int J Immunogenet Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 Blackwell Publishing Ltd. |
Affiliation:
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Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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