Document Detail


Comprehensive characterization of two different Nicotiana tabacum cell lines leads to doubled GFP and HA protein production by media optimization.
MedLine Citation:
PMID:  22055919     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
For over two decades, plant cell cultures have been a promising research platform to express recombinant and therapeutic proteins such as hormones, growth factors, full-size antibodies and antigens. Chosen as a good host for manufacturing recombinant proteins, the Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cell line has been studied in shake flasks by offline analysis of only a few growth parameters. The objective of this study is to comprehensively characterize the growth of a transgenic BY-2 cell line and to investigate the expression profile of the model protein GFP. Based on the correlations between nutrient consumption, cell growth and product formation, the intention is to improve the standard MS-medium. Hereby, multiple growth parameters were analyzed offline and online by using a Respiration Activity monitoring system (RAMOS). A reproducibly observed shift of the oxygen transfer rate (OTR) could be identified to indicate ammonium depletion in the medium. Concurrent with this ammonium depletion, the total protein concentration began to decrease. After the MS-medium was improved, the GFP concentration nearly doubled. When this improved ammonium enriched medium was applied to another transgenic tobacco cell line similar improvements to the amount of the glycoprotein influenza hemagglutinin (HA) produced by Nicotiana tabacum NT-1 cells could be achieved. Ultimately, this combined offline and online analysis can be successfully used for further cell line characterization and media optimization to improve growth and boost target product formation.
Authors:
David A Ullisch; Christina A Müller; Sabrina Maibaum; Janina Kirchhoff; Andreas Schiermeyer; Stefan Schillberg; Jean L Roberts; Wiltrud Treffenfeldt; Jochen Büchs
Related Documents :
21786169 - Valproic acid induced differentiation and potentiated efficacy of taxol and nanotaxol f...
368029 - Bacteriophage t4d receptors and the escherichia coli cell wall structure: role of spher...
7749719 - Correlationships between enzymatic activity of lectins, putrescine content and chloropl...
12238949 - Significant quantities of the glycolytic enzyme phosphoglycerate mutase are present in ...
10407279 - A large-scale sonication assay for cell wall mutant analysis in yeast.
6841579 - Aeromonas hydrophila typing scheme based on patterns of agglutination with erythrocytes...
11715019 - Cooperative regulation of ajm-1 controls junctional integrity in caenorhabditis elegans...
17167749 - Pharmacological approaches to nitric oxide signalling during neural development of locu...
22768029 - Dir-labeled embryonic stem cells for targeted imaging of in vivo gastric cancer cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-4
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  -     ISSN:  1347-4421     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
RWTH Aachen University, AVT - Biochemical Engineering, Worringer Weg 1, 52056 Aachen, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Transgenic rice accumulating modified cedar pollen allergen Cry j 2 derivatives.
Next Document:  Hypergravity effects on myoblast proliferation and differentiation.