| Comprehensive analysis of differential gene expression profiles on diclofenac-induced acute mouse liver injury and recovery. | |
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MedLine Citation:
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PMID: 16859844 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microarray analysis of RNA from diclofenac-administered mouse livers was performed to establish a global gene expression profile during injury and recovery stages at two different doses. A single dose of diclofenac at 9.5 mg/kg or 0.95 mg/kg body weight was given orally, and the liver samples were obtained after 6, 24, and 72 h. Histopathologic studies enabled the classification of the diclofenac effect into injury (6, 24 h) and recovery (72 h) stages. By using the Applied Biosystems Mouse Genome Survey Microarray, a total of 7370 out of 33,315 (22.1%) genes were found to be statistically reliable at p<0.05 by two-way ANOVA, and 602 (1.8%) probes at false discovery rate <5% by Significance Analysis of Microarray. Among the statistically reliable clones by both analytical methods, 49 genes were differentially expressed with more than a 1.625-fold difference (which equals 0.7 in log(2) scale) at one or more treatment conditions. Forty genes and two genes were identified as injury- and recovery-specific genes, respectively, showing that most of the transcriptomic changes were seen during the injury stage. Furthermore, multiple genes involved in oxidative stress, eicosanoid synthesis, apoptosis, and ATP synthesis showed variable transcript levels upon acute diclofenac administration. |
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Authors:
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Heekyoung Chung; Hyun-Jun Kim; Ki-Seok Jang; Mingoo Kim; Jungeun Yang; Ju Han Kim; Yong-Sung Lee; Gu Kong |
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Publication Detail:
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Type: Journal Article Date: 2006-06-12 |
Journal Detail:
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Title: Toxicology letters Volume: 166 ISSN: 0378-4274 ISO Abbreviation: Toxicol. Lett. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-28 Completed Date: 2006-10-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 77-87 Citation Subset: IM |
Affiliation:
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Department of Pathology, College of Medicine, Hanyang University, Seongdong-gu, Seoul 133-791, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents, Non-Steroidal / adverse effects* Diclofenac / adverse effects* Drug-Induced Liver Injury / etiology*, genetics, pathology Gene Expression / drug effects* Gene Expression Profiling Liver / drug effects*, metabolism, pathology Liver Regeneration / genetics* Male Mice Mice, Inbred ICR Oligonucleotide Array Sequence Analysis Oxidative Stress / drug effects, genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 15307-86-5/Diclofenac |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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