Document Detail


Comprehensive analyses of prostate gene expression: convergence of expressed sequence tag databases, transcript profiling and proteomics.
MedLine Citation:
PMID:  10870968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several methods have been developed for the comprehensive analysis of gene expression in complex biological systems. Generally these procedures assess either a portion of the cellular transcriptome or a portion of the cellular proteome. Each approach has distinct conceptual and methodological advantages and disadvantages. We have investigated the application of both methods to characterize the gene expression pathway mediated by androgens and the androgen receptor in prostate cancer cells. This pathway is of critical importance for the development and progression of prostate cancer. Of clinical importance, modulation of androgens remains the mainstay of treatment for patients with advanced disease. To facilitate global gene expression studies we have first sought to define the prostate transcriptome by assembling and annotating prostate-derived expressed sequence tags (ESTs). A total of 55000 prostate ESTs were assembled into a set of 15953 clusters putatively representing 15953 distinct transcripts. These clusters were used to construct cDNA microarrays suitable for examining the androgen-response pathway at the level of transcription. The expression of 20 genes was found to be induced by androgens. This cohort included known androgen-regulated genes such as prostate-specific antigen (PSA) and several novel complementary DNAs (cDNAs). Protein expression profiles of androgen-stimulated prostate cancer cells were generated by two-dimensional electrophoresis (2-DE). Mass spectrometric analysis of androgen-regulated proteins in these cells identified the metastasis-suppressor gene NDKA/nm23, a finding that may explain a marked reduction in metastatic potential when these cells express a functional androgen receptor pathway.
Authors:
P S Nelson; D Han; Y Rochon; G L Corthals; B Lin; A Monson; V Nguyen; B R Franza; S R Plymate; R Aebersold; L Hood
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Electrophoresis     Volume:  21     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1823-31     Citation Subset:  IM    
Affiliation:
Fred Hutchinson Cancer Research Center, Department of Molecular Biotechnology, University of Washington, Seattle, USA. pnelson@fhcrc.org
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MeSH Terms
Descriptor/Qualifier:
DNA, Complementary
Databases, Factual*
Expressed Sequence Tags
Gene Expression*
Gene Expression Profiling / methods
Humans
Male
Mass Spectrometry
Oligonucleotide Array Sequence Analysis
Prostate / metabolism*
Proteome / genetics*
Grant Support
ID/Acronym/Agency:
CA75173/CA/NCI NIH HHS; CRR11823/RR/NCRR NIH HHS; R01-DK52683/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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