| Comprehensive Quality Evaluation of Corn Steep Liquor in 2-Keto-L-Gulonic Acid Fermentation. | |
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MedLine Citation:
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PMID: 21793578 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Corn steep liquor (CSL) is one of the main raw materials in 2-keto-L-gulonic acid (2-KLG) fermentation by Ketogulonicigenium vulgare and Bacillus megaterium. Due to its natural origin, and variations in the manufacturing process, unpredicted and uncontrolled variability of CSL have a great influence on the 2-KLG production, however, conventional quality specifications are not enough to ensure stability of fermentation behaviors. A process analytical technology (PAT) could be considered to explore the relationship between CSL quality and 2-KLG production comprehensively. The compositions of CSL from six manufactures were profiled by gas chromatography with time-of-flight mass spectrometry (GC-TOFMS) and inductively coupled plasma-atomic emission spectroscopy (ICP-AES), combined with orthogonal partial least squares discriminant analysis (OPLS-DA). Seventeen components were identified as the most discriminant marker compounds related to 2-KLG production. Results revealed that they were responsible for providing nutrients and protecting osmotic pressure. We further verified nine amino acids as potential group markers by adding to the medium and demonstrated the correlation to 2-KLG production. The comprehensive approach provided an important platform to explore CSL marker compounds for quality evaluation in 2-KLG fermentation. |
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Authors:
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Yun Gao; Yingjin Yuan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-27 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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