Document Detail

Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung.
MedLine Citation:
PMID:  22674387     Owner:  NLM     Status:  MEDLINE    
During pregnancy, cells from each fetus travel into the maternal circulation and organs, resulting in the development of microchimerism. Identification of the cell types in this microchimeric population would permit better understanding of possible mechanisms by which they affect maternal health. However, comprehensive analysis of fetal cells has been hampered by their rarity. In this study, we sought to overcome this obstacle by combining flow cytometry with multidimensional gene expression microarray analysis of fetal cells isolated from the murine maternal lung during late pregnancy. Fetal cells were collected from the lungs of pregnant female mice. cDNA was amplified and hybridized to gene expression microarrays. The resulting fetal cell core transcriptome was interrogated using multiple methods including Ingenuity Pathway Analysis, the BioGPS gene expression database, principal component analysis, the Eurexpress gene expression atlas, and primary literature. Here we report that small numbers of fetal cells can be flow sorted from the maternal lung, facilitating discovery-driven gene expression analysis. We additionally show that gene expression data can provide functional information about fetal cells. Our results suggest that fetal cells in the murine maternal lung are a mixed population, consisting of trophoblasts, mesenchymal stem cells, and cells of the immune system. Detection of trophoblasts and immune cells in the maternal lung may facilitate future mechanistic studies related to the development of immune tolerance and pregnancy-related complications, such as pre-eclampsia. Furthermore, the presence and persistence of mesenchymal stem cells in maternal organs may have implications for long-term postpartum maternal health.
Stephanie Pritchard; Heather C Wick; Donna K Slonim; Kirby L Johnson; Diana W Bianchi
Related Documents :
8919517 - Changes in skin ph after the use of baby wipes.
21247637 - Dual purpose contraceptives: targeting fertility and sexually transmitted disease.
3910207 - Experimental congenital syphilis in rabbits.
24395147 - Correlation between prenatal ultrasound and fetal autopsy findings on urinary system an...
18264967 - Adam12s in maternal serum as a potential marker of pre-eclampsia.
16400077 - Clinical use of pentoxifylline for activation of immotile testicular sperm before icsi ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-23
Journal Detail:
Title:  Biology of reproduction     Volume:  87     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-24     Completed Date:  2013-01-02     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  42     Citation Subset:  IM    
Mother Infant Research Institute, Floating Hospital at Tufts Medical Center, Boston, Massachusetts, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Flow Cytometry
Gene Expression Profiling
Lung / cytology*
Mesenchymal Stromal Cells / cytology
Mice, Inbred C57BL
Nucleic Acid Amplification Techniques
Placenta / cytology
Pregnancy, Animal* / immunology
Principal Component Analysis
Grant Support
R01 HD-049469-05/HD/NICHD NIH HHS; R01 HD-058880/HD/NICHD NIH HHS; T32 HD-049341-05/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  E-health: easing translation in health care.
Next Document:  The Anti-Apoptotic Effect of Galectin-3 in Human Endometrial Cells under the Regulation of Estrogen ...