Document Detail

Comprehensive analysis of LC/MS data using pseudocolor plots.
MedLine Citation:
PMID:  23283727     Owner:  NLM     Status:  MEDLINE    
We have developed new applications of the pseudocolor plot for the analysis of LC/MS data. These applications include spectral averaging, analysis of variance, differential comparison of spectra, and qualitative filtering by compound class. These applications have been motivated by the need to better understand LC/MS data generated from analysis of human biofluids. The examples presented use data generated to profile steroid hormones in urine extracts from a Cushing's disease patient relative to a healthy control, but are general to any discovery-based scanning mass spectrometry technique. In addition to new visualization techniques, we introduce a new metric of variance: the relative maximum difference from the mean. We also introduce the concept of substructure-dependent analysis of steroid hormones using precursor ion scans. These new analytical techniques provide an alternative approach to traditional untargeted metabolomics workflow. We present an approach to discovery using MS that essentially eliminates alignment or preprocessing of spectra. Moreover, we demonstrate the concept that untargeted metabolomics can be achieved using low mass resolution instrumentation.
Christopher A Crutchfield; Matthew T Olson; Evgenia Gourgari; Maria Nesterova; Constantine A Stratakis; Alfred L Yergey
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2013-01-03
Journal Detail:
Title:  Journal of the American Society for Mass Spectrometry     Volume:  24     ISSN:  1879-1123     ISO Abbreviation:  J. Am. Soc. Mass Spectrom.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-11     Completed Date:  2013-07-22     Revised Date:  2014-08-27    
Medline Journal Info:
Nlm Unique ID:  9010412     Medline TA:  J Am Soc Mass Spectrom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  230-7     Citation Subset:  IM    
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MeSH Terms
Analysis of Variance
Case-Control Studies
Chromatography, High Pressure Liquid / methods*
Hormones / urine
Image Processing, Computer-Assisted / methods*
Mass Spectrometry / methods*
Metabolomics / methods
Pituitary ACTH Hypersecretion / urine
Steroids / urine
Grant Support
Reg. No./Substance:
0/Hormones; 0/Steroids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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