Document Detail


Compositional differences between infant and adult human corneal basement membranes.
MedLine Citation:
PMID:  17962449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Adult human corneal epithelial basement membrane (EBM) and Descemet's membrane (DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development.
METHODS: Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components.
RESULTS: Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha1-alpha2 to alpha3-alpha4 chains after 3 years of life; in the adult, alpha1-alpha2 chains were retained only in the limbal BM. Laminin alpha2 and beta2 chains were present in the adult limbal BM where epithelial stem cells are located. By 3 years of age, beta2 chain appeared in the limbal BM. In all corneas, limbal BM contained laminin gamma3 chain. In the infant DM, type IV collagen alpha1-alpha6 chains, perlecan, nidogen-1, nidogen-2, and netrin-4 were found on both faces, but they remained only on the endothelial face of the adult DM. The stromal face of the infant but not the adult DM was positive for tenascin-C, fibrillin-1, SPARC, and laminin-332. Type VIII collagen shifted from the endothelial face of infant DM to its stromal face in the adult. Matrilin-4 largely disappeared after the age of 3 years.
CONCLUSIONS: The distribution of laminin gamma3 chain, nidogen-2, netrin-4, matrilin-2, and matrilin-4 is described in the cornea for the first time. The observed differences between adult and infant corneal BMs may relate to changes in their mechanical strength, corneal cell adhesion and differentiation in the process of postnatal corneal maturation.
Authors:
Andrea Kabosova; Dimitri T Azar; Gregory A Bannikov; Kevin P Campbell; Madeleine Durbeej; Reza F Ghohestani; Jonathan C R Jones; M Cristina Kenney; Manuel Koch; Yoshifumi Ninomiya; Bruce L Patton; Mats Paulsson; Yoshikazu Sado; E Helene Sage; Takako Sasaki; Lydia M Sorokin; Marie-France Steiner-Champliaud; Tung-Tien Sun; Nirmala Sundarraj; Rupert Timpl; Ismo Virtanen; Alexander V Ljubimov
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  48     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-26     Completed Date:  2007-12-06     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4989-99     Citation Subset:  IM    
Affiliation:
Ophthalmology Research Laboratories, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Monoclonal
Basement Membrane / chemistry*
Bowman Membrane / chemistry*
Child, Preschool
Descemet Membrane / chemistry*
Extracellular Matrix Proteins / analysis*
Humans
Infant
Infant, Newborn
Microscopy, Fluorescence
Middle Aged
Grant Support
ID/Acronym/Agency:
R01 AR054184-18/AR/NIAMS NIH HHS; R01 AR054184-20/AR/NIAMS NIH HHS; R01 EY013431/EY/NEI NIH HHS; R01 EY013431-06/EY/NEI NIH HHS; R01 EY10836/EY/NEI NIH HHS; R01 EY13431/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Extracellular Matrix Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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