| Composition and structure of triacylglycerols in brown adipose tissue of rats fed fish oil. | |
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MedLine Citation:
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PMID: 7869856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examines the incorporation of highly unsaturated n-3 fatty acids (HUFA) into triacylglycerols (TAG) of brown adipose tissue (BAT), and their effect on the positional distribution of saturated (SFA) and of unsaturated (UFA) 16- or 18-carbon fatty acids. To this end, rats were fed a fish oil diet for up to four weeks. The stereospecific analysis of TAG was based on generation of sn-1,2- and sn-2,3-acylglycerols by Grignard degradation, followed by synthesis of phosphatidic acid and specific hydrolysis with phospholipase A2. From the end of the first week of fish oil feeding, a steady-state in the fatty acid composition of TAG in BAT was reached. HUFA concentration increased 30-fold, mainly at the expense of n-9 UFA and of SFA. The amount of SFA decreased selectively at position 3, where these fatty acids were progressively replaced by n-3 HUFA. By contrast, the amount of UFA decreased at all positions, and their positional distribution was not affected. About 60% of HUFA was incorporated at position 3. Nearly twice as much 22:6n-3 was incorporated into TAG than had been previously observed in white adipose tissue (WAT) [Leray, C., Raclot, T., and Groscolas, R. (1993) Lipids 28, 279-284]. At the steady-state, the distribution of HUFA was characterized by high proportions of 22:6n-3 and 20:5n-3 in position 3. Moreover, in each position of TAG, a steady level was reached rapidly (within 1 wk).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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T Raclot; R Groscolas; C Leray |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Lipids Volume: 29 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1995-03-30 Completed Date: 1995-03-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 759-64 Citation Subset: IM |
Affiliation:
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Centre d'Ecologie et Physiologie Energétiques, Strasbourg, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue, Brown
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chemistry* Animal Feed Animals Fatty Acids, Omega-3 / chemistry, metabolism Fatty Acids, Unsaturated / chemistry, metabolism Fish Oils / pharmacology* Male Rats Rats, Wistar Triglycerides / analysis, chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Omega-3; 0/Fatty Acids, Unsaturated; 0/Fish Oils; 0/Triglycerides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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