Document Detail


Composition and structure of triacylglycerols in brown adipose tissue of rats fed fish oil.
MedLine Citation:
PMID:  7869856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examines the incorporation of highly unsaturated n-3 fatty acids (HUFA) into triacylglycerols (TAG) of brown adipose tissue (BAT), and their effect on the positional distribution of saturated (SFA) and of unsaturated (UFA) 16- or 18-carbon fatty acids. To this end, rats were fed a fish oil diet for up to four weeks. The stereospecific analysis of TAG was based on generation of sn-1,2- and sn-2,3-acylglycerols by Grignard degradation, followed by synthesis of phosphatidic acid and specific hydrolysis with phospholipase A2. From the end of the first week of fish oil feeding, a steady-state in the fatty acid composition of TAG in BAT was reached. HUFA concentration increased 30-fold, mainly at the expense of n-9 UFA and of SFA. The amount of SFA decreased selectively at position 3, where these fatty acids were progressively replaced by n-3 HUFA. By contrast, the amount of UFA decreased at all positions, and their positional distribution was not affected. About 60% of HUFA was incorporated at position 3. Nearly twice as much 22:6n-3 was incorporated into TAG than had been previously observed in white adipose tissue (WAT) [Leray, C., Raclot, T., and Groscolas, R. (1993) Lipids 28, 279-284]. At the steady-state, the distribution of HUFA was characterized by high proportions of 22:6n-3 and 20:5n-3 in position 3. Moreover, in each position of TAG, a steady level was reached rapidly (within 1 wk).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
T Raclot; R Groscolas; C Leray
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Lipids     Volume:  29     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-03-30     Completed Date:  1995-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  759-64     Citation Subset:  IM    
Affiliation:
Centre d'Ecologie et Physiologie Energétiques, Strasbourg, France.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue, Brown / chemistry*
Animal Feed
Animals
Fatty Acids, Omega-3 / chemistry,  metabolism
Fatty Acids, Unsaturated / chemistry,  metabolism
Fish Oils / pharmacology*
Male
Rats
Rats, Wistar
Triglycerides / analysis,  chemistry*
Chemical
Reg. No./Substance:
0/Fatty Acids, Omega-3; 0/Fatty Acids, Unsaturated; 0/Fish Oils; 0/Triglycerides

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