Document Detail


Composition of phospholipid fatty acids in erythrocyte membranes of children with chronic juvenile arthritis: clinical and biochemical correlations.
MedLine Citation:
PMID:  11208280     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Systemic diseases of connective tissue, including chronic juvenile arthritis are associated with a number of metabolic disorders such as e.g. lipid disturbances. The purpose of the present work was to analyse the composition of fatty acids in erythrocyte phospholipids of children with juvenile chronic arthritis and to determine a correlation between the composition of these acids and patients' clinical status. The study was conducted on 47 children with juvenile chronic arthritis (jca) and 29 healthy subjects. The following fractions of phospholipids were obtained with the help of thin-layer chromatography: phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidyloinositol, cardiolipin, sphingolipin. Fatty acids were analysed with the use of gas chromatograph (Hewlett-Packard 5890). Saturated fatty acids as well as mono- and polyunsaturated (n-3 and n-6) fatty acids were identified. The decrease in the percentage of linolenic acid, PUFA n-6 and PUFA n-3 was found in all the phospholipid fractions in children with jca when compared with control group. There was a concurrent increase in saturated fatty acids, mainly stearic and palmitic acids. The differences in the distribution of fatty acids in erythrocyte phospholipids were observed at early stage of the disease and they became more conspicuous as inflammatory process proceeded.
Authors:
A Górska; A Nawrocki; M Urban; B Florys
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  6     ISSN:  1234-1010     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:    2000 Jan-Feb
Date Detail:
Created Date:  2001-09-11     Completed Date:  2001-10-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  30-9     Citation Subset:  IM    
Affiliation:
2nd Department of Paediatric Diseases, Medical University, ul. Waszyngtona 17, 15-274 Białystok, Poland.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Arthritis, Juvenile Rheumatoid / blood*
Cardiolipins / blood,  chemistry
Case-Control Studies
Child
Erythrocyte Membrane / metabolism*
Fatty Acids / blood*,  chemistry
Female
Humans
Male
Phosphatidylcholines / blood,  chemistry
Phosphatidylethanolamines / blood,  chemistry
Phosphatidylinositols / blood,  chemistry
Phosphatidylserines / blood,  chemistry
Phospholipids / blood*,  chemistry
Chemical
Reg. No./Substance:
0/Cardiolipins; 0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phosphatidylinositols; 0/Phosphatidylserines; 0/Phospholipids

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