| Composition and metabolic activities of bacterial biofilms colonizing food residues in the human gut. | |
| | |
MedLine Citation:
|
PMID: 16957247 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bacteria growing in the human large intestine live in intimate association with the host and play an important role in host digestive processes, gut physiology, and metabolism. Fecal bacteria have been investigated extensively, but few studies have been done on biofilms that form on digestive wastes in the large bowel. The aims of this investigation were to investigate the composition and metabolic activities of bacterial communities that colonize the surfaces of food residues in fecal material, with respect to their role in the fermentation of complex carbohydrates. Fresh stools were obtained from 15 healthy donors, and food residues were separated by filtration. Adherent bacteria were removed by surfactant treatment for microbiological analysis and fermentation studies. Scanning electron microscopy and fluorescent in situ hybridization in conjunction with confocal laser scanning microscopy (CLSM) were used to visualize intact biofilms. Results showed that bacterial populations strongly adhering to particulate matter were phenotypically similar in composition to unattached communities, with bacteroides and bifidobacteria predominating. Biofilms comprised a mixture of living and dead bacteria, and CLSM showed that bifidobacteria in the biofilms occurred as isolated dispersed cells and in microcolonies near the interface with the substratum. Fermentation experiments with a variety of complex carbohydrates demonstrated that biofilm populations were more efficient in digesting polysaccharides, while nonadhering communities fermented oligosaccharides most rapidly. Acetate was the principal fermentation product formed by biofilm bacteria, whereas higher levels of butyrate were produced by nonadherent populations, showing that the two communities were metabolically distinct. |
| | |
Authors:
|
Sandra Macfarlane; George T Macfarlane |
Related Documents
:
|
22911977 - Obesity and fast food in urban markets: a new approach using geo-referenced micro data. 20412607 - Changes in starch physical characteristics following digestion of foods in the human sm... 15672227 - Production of poly(3-hydroxybutyrate) by solid-state fermentation with ralstonia eutropha. 2853047 - Comparison of the effects on colonic function caused by feeding rice bran and wheat bran. 17367877 - Fasting increases and satiation decreases olfactory detection for a neutral odor in rats. 15032127 - Functional foods and the urinary tract. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 72 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2006 Sep |
Date Detail:
|
Created Date: 2006-09-07 Completed Date: 2006-11-08 Revised Date: 2013-06-07 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 6204-11 Citation Subset: IM |
Affiliation:
|
Gut Group, University of Dundee, Level 6, Ninewells Hospital Medical School, Dundee DD1 9SY, United Kingdom. s.macfarlane@dundee.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteria
/
genetics,
isolation & purification,
metabolism* Base Sequence Biofilms / growth & development* Carbohydrate Metabolism DNA Probes / genetics DNA, Bacterial / genetics Digestive System / microbiology* Feces / microbiology Fermentation Food Microbiology* Humans In Situ Hybridization, Fluorescence Microscopy, Confocal Microscopy, Electron, Scanning |
| Chemical | |
Reg. No./Substance:
|
0/DNA Probes; 0/DNA, Bacterial |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Global transcriptional and physiological responses of Saccharomyces cerevisiae to ammonium, L-alanin...
Next Document: Elucidation of the hrp clusters of Xanthomonas oryzae pv. oryzicola that control the hypersensitive ...