Document Detail


Composition and characterization of an acidic polysaccharide from out-of-date tea leaves.
MedLine Citation:
PMID:  22019982     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An acidic polysaccharide conjugate from the tea leaves (Camellia sinensis) beyond the useful date was isolated and characterized. The crude polysaccharides (TPS) were extracted with hot water and separated on a DEAE sepharose FF gel filtration column to obtain ATPS2. The chemical structure of ATPS2 was investigated by GC, IC, HPGPC, IR, UV, Periodate oxidation-Smith degradation, (1)H NMR, and (13)C NMR methods and its conformation was observed with Congo red test. The crude TPS mainly consisted of 5 peaks with molecular weights of 7.67×10(5), 2.03×10(5), 2.83×10(4), 3470.2, and 1205.52Da, respectively. The molecular weight of ATPS2 was determined as 7.4×10(5)Da by HPGPC. IC chromatogram of ATPS2 showed that ATPS2 consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose and galacturonic acid. Compared with the crude TPS, ribose and glucuronic acid were not found in ATPS2. Periodate oxidation-Smith degradation results showed that ATPS2 is a linear (1→3) β-d-Glc main chain linkage with 1→ or 1→6-linked chains and there are no 1→2 or 1→4-linked chains. The main glycosyl residues of ATPS2 were α-, β-configuration, including signal of β-d-Galp, α-l-Araf, α-d-Manp, α-Rhap, and α-d-Glcp. Congo red test exhibited that ATPS2 did not have a triple-helical conformation.
Authors:
Jianbo Xiao; Jianglei Huo; Huixian Jiang; Koichiro Yamamoto
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-17
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Affiliation:
College of Life & Environment Science, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, PR China.
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