| Composition and characterization of an acidic polysaccharide from out-of-date tea leaves. | |
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MedLine Citation:
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PMID: 22019982 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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An acidic polysaccharide conjugate from the tea leaves (Camellia sinensis) beyond the useful date was isolated and characterized. The crude polysaccharides (TPS) were extracted with hot water and separated on a DEAE sepharose FF gel filtration column to obtain ATPS2. The chemical structure of ATPS2 was investigated by GC, IC, HPGPC, IR, UV, Periodate oxidation-Smith degradation, (1)H NMR, and (13)C NMR methods and its conformation was observed with Congo red test. The crude TPS mainly consisted of 5 peaks with molecular weights of 7.67×10(5), 2.03×10(5), 2.83×10(4), 3470.2, and 1205.52Da, respectively. The molecular weight of ATPS2 was determined as 7.4×10(5)Da by HPGPC. IC chromatogram of ATPS2 showed that ATPS2 consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose and galacturonic acid. Compared with the crude TPS, ribose and glucuronic acid were not found in ATPS2. Periodate oxidation-Smith degradation results showed that ATPS2 is a linear (1→3) β-d-Glc main chain linkage with 1→ or 1→6-linked chains and there are no 1→2 or 1→4-linked chains. The main glycosyl residues of ATPS2 were α-, β-configuration, including signal of β-d-Galp, α-l-Araf, α-d-Manp, α-Rhap, and α-d-Glcp. Congo red test exhibited that ATPS2 did not have a triple-helical conformation. |
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Authors:
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Jianbo Xiao; Jianglei Huo; Huixian Jiang; Koichiro Yamamoto |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-17 |
Journal Detail:
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Title: International journal of biological macromolecules Volume: - ISSN: 1879-0003 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
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College of Life & Environment Science, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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