| Composition of the GABA(A) receptors of retinal dopaminergic neurons. | |
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MedLine Citation:
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PMID: 10479684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transgenic technology, single-cell RT-PCR, and immunocytochemistry were combined to investigate the composition of the GABA(A) receptors of dopaminergic (interplexiform) amacrine (DA) cells. A mouse line was used in which these neurons were labeled with human placental alkaline phosphatase and could therefore be identified in vitro after dissociation of the retina. We performed single-cell RT-PCR on the isolated cells and showed that (1) DA cells contained the messages for alpha1, alpha3, alpha4, beta1, beta3, gamma1, gamma2(S), and gamma2(L) subunits; (2) this transcript repertory did not change on dissociation of the retina and throughout the time required for cell harvesting; and (3) all DA cells contained the entire transcript repertory. Immunocytochemistry with subunit-specific antibodies showed that all subunits were expressed and appeared homogeneously distributed throughout the cell membrane at a low concentration. In addition, with the exception of alpha4, the subunits formed clusters at the surface of the dendrites and on the inner pole of the cell body. Because of their size, shape, and topographic coincidence with GABAergic endings, the clusters were interpreted as postsynaptic active zones containing GABA(A) receptors. The composition of the synaptic receptors was not uniform: clusters distributed throughout the dendritic tree contained alpha3, beta3, and, less frequently, beta1 subunits, whereas clusters containing the alpha1 subunit were confined to large dendrites. Therefore, DA cells possess at least two types of GABA(A) receptors localized in different synapses. Furthermore, they exhibit multiple extrasynaptic GABA(A) receptors. |
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Authors:
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S Gustincich; A Feigenspan; W Sieghart; E Raviola |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 19 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-10-04 Completed Date: 1999-10-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7812-22 Citation Subset: IM |
Affiliation:
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Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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analysis,
genetics Animals Dopamine / analysis Humans Immunohistochemistry Macromolecular Substances Mice Mice, Transgenic Neurons / cytology, physiology* Receptors, GABA-A / analysis*, chemistry, genetics* Restriction Mapping Retina / cytology, physiology* Reverse Transcriptase Polymerase Chain Reaction Synapses / physiology, ultrastructure Transcription, Genetic* Tyrosine 3-Monooxygenase / analysis, genetics |
| Grant Support | |
ID/Acronym/Agency:
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EY-01344/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 0/Receptors, GABA-A; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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