Document Detail


Composite interval mapping reveals a major locus influencing embryonic development rate in rainbow trout (Oncorhynchus mykiss).
MedLine Citation:
PMID:  11336224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Little is known about the genetics controlling the rate of embryonic development in salmonids, despite the fact that this trait plays an important role in the life history of wild and cultured stocks. We investigated the genetics of embryonic development rate by performing an analysis of quantitative trait loci (QTL) on two families of androgenetically derived doubled haploid rainbow trout produced from a hybrid of two clonal lines with divergent embryonic development rates. A total of 170 doubled haploid individuals were genotyped at 222 marker loci [219 amplified fragment length polymorphism (AFLP) markers, 2 microsatellites, and p53]. A genetic linkage analysis resulted in a map consisting of 27 linkage groups with 21 of the markers remaining unlinked at a minimum LOD of 3.0 and maximum theta of 0.40. Eight of these linkage groups were matched to published rainbow trout linkage groups. Composite interval mapping (CIM) revealed evidence for two QTL influencing time to hatch, and suggestive evidence for a third. These QTL accounted for a total of 24.6% of the variation in time to hatch. One of these QTL had a large effect on development rate, especially in one family of doubled haploids, in which it explained 25.6% of the variance in time to hatch. QTL influencing embryonic length and weight at the commencement of exogenous feeding were also identified. The QTL with the strongest effect on embryonic length (lenR13) mapped to the same position as the QTL with the strongest effect on time to hatch (tthR13), suggesting a single QTL may have a pleiotropic effect on both these traits. These results suggest that the use of clonal lines with a doubled haploid crossing design is an effective way of analyzing the genetic basis of complex traits in salmonids.
Authors:
B D Robison; P A Wheeler; K Sundin; P Sikka; G H Thorgaard
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of heredity     Volume:  92     ISSN:  0022-1503     ISO Abbreviation:  J. Hered.     Publication Date:    2001 Jan-Feb
Date Detail:
Created Date:  2001-05-04     Completed Date:  2001-09-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375373     Medline TA:  J Hered     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16-22     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, Washington State University, Pullman, USA. barrie@darkwing.uoregon.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Mapping*
Female
Fetal Weight / genetics*,  physiology
Genes, Dominant
Lod Score
Male
Microsatellite Repeats
Oncorhynchus mykiss / genetics*
Polymorphism, Restriction Fragment Length
Quantitative Trait, Heritable*
Grant Support
ID/Acronym/Agency:
1 RO1-RR06654/RR/NCRR NIH HHS; P01 ES04766/ES/NIEHS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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