Document Detail

Components of the renin-angiotensin system in adipose tissue: changes with maturation and adipose mass enlargement.
MedLine Citation:
PMID:  7671018     Owner:  NLM     Status:  MEDLINE    
White adipose tissue is a rich source of angiotensinogen protein and mRNA. Studies in clonal cells suggest that angiotensinogen, and its cleavage product, angiotensin II, are involved in preadipocyte differentiation into mature fat cells. No studies have determined whether angiotensinogen is also involved in adipose tissue development in vivo. In this report, we studied male Wistar rats at two stages of development to determine if angiotensinogen protein and mRNA are increased in retroperitoneal fat depots of rapidly growing young, lean, 8-week-old rats compared to 26-week-old rats that are fatter, but are undergoing less rapid adipose tissue growth. We also assessed renin mRNA and angiotensin I-generating activity, since it is less clear whether renin is locally produced in adipose tissue. We found that angiotensin I-generating activity was measurable in adipose tissue and adipocytes, but renin mRNA was undetectable by northern blot analysis. Angiotensinogen mRNA was abundant in adipocytes, but was absent in stromal-vascular cells of adipose tissue. Angiotensinogen content per 10 million fat cells was approximately threefold higher in 8-week-old rats compared to 26-week-old rats (p < .0002). Angiotensinogen mRNA was approximately twofold higher in adipocytes of 8-week-old rats compared to 26-week-old rats. The age-related decline in angiotensinogen protein and mRNA indicates that the local renin-angiotensin system may play an important role in adipose tissue growth, and possibly contribute to the changes in adipose mass and cellularity seen in old senescent rats.
J B Harp; M DiGirolamo
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journals of gerontology. Series A, Biological sciences and medical sciences     Volume:  50     ISSN:  1079-5006     ISO Abbreviation:  J. Gerontol. A Biol. Sci. Med. Sci.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-10-18     Completed Date:  1995-10-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9502837     Medline TA:  J Gerontol A Biol Sci Med Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  B270-6     Citation Subset:  AIM; IM    
Department of Medicine, Emory University School of Medicine, Atlanta, USA.
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MeSH Terms
Adipocytes / metabolism
Adipose Tissue / growth & development*,  metabolism*
Aging / metabolism*
Angiotensinogen / genetics,  metabolism
Blotting, Northern
Body Composition
Body Weight
RNA, Messenger / metabolism
Rats, Wistar
Renin / genetics,  metabolism
Renin-Angiotensin System*
Grant Support
1 F32 DK08346/DK/NIDDK NIH HHS; 3 M01 RR00039-31S/RR/NCRR NIH HHS
Reg. No./Substance:
0/RNA, Messenger; 11002-13-4/Angiotensinogen; EC

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