Document Detail


Component-resolved diagnosis with recombinant allergens in patients with cherry allergy.
MedLine Citation:
PMID:  12110837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In pollen-related food allergy, extracts for skin prick tests (SPTs) are often not standardized, and the test reliability is affected by false-negative reactions. OBJECTIVE: We sought to evaluate a panel of recombinant allergens (RAs) derived from one allergenic food for use in component-resolved in vivo diagnosis, taking cherry as a model food. METHODS: Seventy-nine subjects were included in the study: 24 Swiss patients (group 1) with a positive double-blind placebo-controlled food challenge result to cherries, 23 patients with birch pollen allergy but without cherry allergy (group 2), 23 nonatopic subjects (group 3), and 9 Spanish patients with a history of a cherry allergy (group 4). SPTs were performed in duplicate by using recombinant cherry allergens (Bet v 1-related allergen: recombinant (r) Pru av 1; profilin: rPru av 4; and lipid transfer protein: rPru av 3) in concentrations of 10, 50, and 100 microg/mL. Furthermore, IgE reactivity to rPru av 1, rPru av 4, and rPru av 3 was assessed by means of immunoblot analysis. RESULTS: SPT responses with rPru av 1, rPru av 4, and rPru av 3 were positive in 92%, 17%, and 4% of the patients in group 1; in 74%, 30%, and 0% of the patients in group 2; in 0%, 22%, and 89% of the patients in group 4; and negative for all nonatopic subjects (group 3). Thus the sensitivity of a positive SPT response to at least one of the 3 RAs was 96%. The specificities, negative predictive values, and positive predictive values with the 3 RAs were 100%, 96%, and 100% if calculated in relation to the nonatopic control group but 17%, 79%, and 60% when calculated in relation to the control group with birch pollen allergy. The correlation between SPT and immunoblotting results was excellent. Sensitization to rPru av 3 was associated with more severe symptoms than sensitization to rPru av 1. CONCLUSIONS: SPTs with RAs proved to be highly sensitive for diagnosis of cherry allergy. Component-resolved in vivo diagnosis with standardized amounts of stable RAs allows us to determine sensitization patterns directly, to correlate them with severity of clinical symptoms, and to analyze geographic differences.
Authors:
Barbara K Ballmer-Weber; Stephan Scheurer; Philipp Fritsche; Ernesto Enrique; Anna Cistero-Bahima; Tanja Haase; Brunello Wüthrich
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  110     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-11     Completed Date:  2002-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-73     Citation Subset:  AIM; IM    
Affiliation:
Allergy Unit, Department of Dermatology, University Hospital, Zurich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Allergens / diagnostic use*,  genetics,  immunology
Betula / immunology
Child
Cross Reactions
Double-Blind Method
Female
Food Hypersensitivity / diagnosis*
Humans
Immunoblotting
Immunoglobulin E / blood
Male
Middle Aged
Plant Proteins / diagnostic use*,  genetics,  immunology
Pollen / immunology
Prunus / adverse effects,  immunology*
Recombinant Proteins / diagnostic use*,  immunology
Sensitivity and Specificity
Skin Tests
Chemical
Reg. No./Substance:
0/Allergens; 0/PRUA1 protein, Prunus avium; 0/Plant Proteins; 0/Recombinant Proteins; 37341-29-0/Immunoglobulin E

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