| Component-resolved diagnosis with recombinant allergens in patients with cherry allergy. | |
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MedLine Citation:
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PMID: 12110837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In pollen-related food allergy, extracts for skin prick tests (SPTs) are often not standardized, and the test reliability is affected by false-negative reactions. OBJECTIVE: We sought to evaluate a panel of recombinant allergens (RAs) derived from one allergenic food for use in component-resolved in vivo diagnosis, taking cherry as a model food. METHODS: Seventy-nine subjects were included in the study: 24 Swiss patients (group 1) with a positive double-blind placebo-controlled food challenge result to cherries, 23 patients with birch pollen allergy but without cherry allergy (group 2), 23 nonatopic subjects (group 3), and 9 Spanish patients with a history of a cherry allergy (group 4). SPTs were performed in duplicate by using recombinant cherry allergens (Bet v 1-related allergen: recombinant (r) Pru av 1; profilin: rPru av 4; and lipid transfer protein: rPru av 3) in concentrations of 10, 50, and 100 microg/mL. Furthermore, IgE reactivity to rPru av 1, rPru av 4, and rPru av 3 was assessed by means of immunoblot analysis. RESULTS: SPT responses with rPru av 1, rPru av 4, and rPru av 3 were positive in 92%, 17%, and 4% of the patients in group 1; in 74%, 30%, and 0% of the patients in group 2; in 0%, 22%, and 89% of the patients in group 4; and negative for all nonatopic subjects (group 3). Thus the sensitivity of a positive SPT response to at least one of the 3 RAs was 96%. The specificities, negative predictive values, and positive predictive values with the 3 RAs were 100%, 96%, and 100% if calculated in relation to the nonatopic control group but 17%, 79%, and 60% when calculated in relation to the control group with birch pollen allergy. The correlation between SPT and immunoblotting results was excellent. Sensitization to rPru av 3 was associated with more severe symptoms than sensitization to rPru av 1. CONCLUSIONS: SPTs with RAs proved to be highly sensitive for diagnosis of cherry allergy. Component-resolved in vivo diagnosis with standardized amounts of stable RAs allows us to determine sensitization patterns directly, to correlate them with severity of clinical symptoms, and to analyze geographic differences. |
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Authors:
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Barbara K Ballmer-Weber; Stephan Scheurer; Philipp Fritsche; Ernesto Enrique; Anna Cistero-Bahima; Tanja Haase; Brunello Wüthrich |
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Publication Detail:
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Type: Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of allergy and clinical immunology Volume: 110 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-07-11 Completed Date: 2002-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 167-73 Citation Subset: AIM; IM |
Affiliation:
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Allergy Unit, Department of Dermatology, University Hospital, Zurich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Allergens / diagnostic use*, genetics, immunology Betula / immunology Child Cross Reactions Double-Blind Method Female Food Hypersensitivity / diagnosis* Humans Immunoblotting Immunoglobulin E / blood Male Middle Aged Plant Proteins / diagnostic use*, genetics, immunology Pollen / immunology Prunus / adverse effects, immunology* Recombinant Proteins / diagnostic use*, immunology Sensitivity and Specificity Skin Tests |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/PRUA1 protein, Prunus avium; 0/Plant Proteins; 0/Recombinant Proteins; 37341-29-0/Immunoglobulin E |
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