Document Detail

Complexity of polysomal polyadenylated RNA in undifferentiated and differentiated neuroblastoma cells.
MedLine Citation:
PMID:  738279     Owner:  NLM     Status:  MEDLINE    
Using cDNA probes, we have analysed the sequence complexity and the frequency distribution of the polysomal poly(A)-containing RNA from neuroblastoma cells at two different developmental states: either as round, immature neuroblasts, or as differentiated cells exhibiting the morphological properties of mature neurons. The total complexities measured for mRNA from undifferentiated and differentiated cells are identical and correspond to approximately 7000 average-sized sequences of 1750 nucleotides distributed in the same three abundance classes. We have determined the homology between the mRNA populations corresponding to the two developmental states by heterologous cross-hybridization: all the sequence from differentiated cells are present in the polysomes of undifferentiated cells. Conversely, the mRNA from differentiated cells fails to hybridize with about 15% of hybridizable cDNA corresponding to undifferentiated cells. This difference probably results from the disappearance of some mRNA species and may be related to the terminal differentiation of neuroblastoma cells.
A Felsani; F Berthelot; F Gros; B Croizat
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  92     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1978 Dec 
Date Detail:
Created Date:  1979-04-28     Completed Date:  1979-04-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  569-77     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation
Cells, Cultured
DNA / metabolism
Hot Temperature
Molecular Weight
Neuroblastoma / metabolism*
Nucleic Acid Hybridization
Poly A*
RNA, Messenger / metabolism
RNA, Ribosomal / isolation & purification,  metabolism*
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Ribosomal; 24937-83-5/Poly A; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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