Document Detail


Complexity of MAM-6, an epithelial sialomucin associated with carcinomas.
MedLine Citation:
PMID:  2643463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The complexity of epithelial sialomucins was investigated by immunoprecipitation and membrane immunofluorescence, using monoclonal antibodies (MAbs) against MAM-6 and other sialomucins. MAbs against MAM-6 immunoprecipitated from a variety of sources either one or two sialylated glycoproteins with apparent molecular weights of over 400,000 under reducing as well as nonreducing conditions. The electrophoretic mobility of each MAM-6 glycoprotein as isolated from serum, milk, and cell lines of different individuals showed considerable variation. The differences in molecular weight of the MAM-6 glycoproteins were also reflected at the level of MAM-6 precursors which are less heavily glycosylated. Therefore, large differences in apparent molecular weight (150,000 and over) are most likely due to a variable protein backbone. We used this molecular polymorphism to prove that 11 MAbs against different sialomucins, obtained from various investigators, precipitated sialomucins generated from common precursor molecules. The pattern of reactivity of the MAbs with carcinoma cell lines was complex. All but the two MAbs, directed against putative carbohydrate epitopes, immunoprecipitated the precursor molecule from each cell line. However, some of them were unable to immunoprecipitate the mature form of MAM-6 from these cell lines. These results indicate that those epitopes are masked, probably due to cell line- or possibly cell type-dependent variations in glycosylation of the epithelial sialomucin. Even within a single cell line mature molecules with different epitopes were observed. The differential reactivity of the MAbs was confirmed by membrane immunofluorescence. These results show that MAM-6 belongs to a family of epithelial sialomucins with a polymorphic protein backbone and extensive variation in glycosylation.
Authors:
J Hilkens; F Buijs; M Ligtenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  49     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-03-07     Completed Date:  1989-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  786-93     Citation Subset:  IM    
Affiliation:
Division of Tumor Biology, The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis), Amsterdam.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use
Antigen-Antibody Complex
Antigens, Neoplasm / analysis*
Breast Neoplasms / analysis
Cell Line
Female
Fluorescent Antibody Technique
Humans
Immunoassay
Membrane Glycoproteins / immunology,  isolation & purification*
Molecular Weight
Mucin-1
Mucins / analysis*
Sialomucins
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex; 0/Antigens, Neoplasm; 0/Membrane Glycoproteins; 0/Mucin-1; 0/Mucins; 0/Sialomucins

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