Document Detail


Complexities of chromosome landing in a highly duplicated genome: toward map-based cloning of a gene controlling blackleg resistance in Brassica napus.
MedLine Citation:
PMID:  16143600     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The LmR1 locus, which controls seedling resistance to the blackleg fungus Leptosphaeria maculans in the Brassica napus cultivar Shiralee, was positioned on linkage group N7. Fine genetic mapping in a population of 2500 backcross lines identified three molecular markers that cosegregated with LmR1. Additional linkage mapping in a second population colocalized a seedling resistance gene, ClmR1, from the cultivar Cresor to the same genetic interval on N7 as LmR1. Both genes were located in a region that showed extensive inter- and intragenomic duplications as well as intrachromosomal tandem duplications. The tandem duplications seem to have occurred in the Brassica lineage before the divergence of B. rapa and B. oleracea but after the separation of Brassica and Arabidopsis from a common ancestor. Microsynteny was found between the region on N7 carrying the resistance gene and the end of Arabidopsis chromosome 1, interrupted by a single inversion close to the resistance locus. The collinear region in Arabidopsis was assayed for the presence of possible candidate genes for blackleg resistance. These data provided novel insights into the genomic structure and evolution of plant resistance loci and an evaluation of the candidate gene approach using comparative mapping with a model organism.
Authors:
Reinhold Mayerhofer; Kris Wilde; Marion Mayerhofer; Derek Lydiate; Vipan K Bansal; Allen G Good; Isobel A P Parkin
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-02
Journal Detail:
Title:  Genetics     Volume:  171     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-22     Completed Date:  2006-10-06     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1977-88     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Alberta, G-404 Biological Sciences Building, Edmonton, Alberta T6G 2E9, Canada. rmayerho@ualberta.ca
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MeSH Terms
Descriptor/Qualifier:
Ascomycota*
Base Sequence
Blotting, Southern
Brassica napus / genetics*
Chromosome Mapping*
Chromosomes, Plant / genetics*
Computational Biology
Crosses, Genetic
DNA Primers
Evolution, Molecular
Genome, Plant / genetics*
Immunity, Innate / genetics*
Molecular Sequence Data
Plant Diseases / genetics,  microbiology*
Sequence Analysis, DNA
Synteny / genetics
Chemical
Reg. No./Substance:
0/DNA Primers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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