| Complexation of siRNA and pDNA with cationic liposomes: the important aspects in lipoplex preparation. | |
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MedLine Citation:
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PMID: 20072901 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the last two decades, cationic liposomes have been investigated as vehicles for nucleic acids [plasmid DNA (pDNA) and small interfering RNA (siRNA)] delivery in vitro and in vivo. The formation of cationic liposomes-nucleic acids complexes, termed lipoplexes, depends on a number of experimental variables. The quality of the nucleic acid and the cationic liposome as well as the selection of diluents for diluting the concentrated stocks strongly affect the resulting lipoplexes and their efficiency of gene-expression or gene-silencing effect following transfection. In addition, the molar ratio of cationic lipid nitrogen (N) to siRNA or pDNA phosphate (P) (N/P ratio) influences the final characteristics of the lipoplexes, such as size, surface zeta potential, and reproducibility, thereby reflecting their efficiency following transfection. The methods presented in this chapter could be helpful to obtain reliable and reproducible lipoplexes and experimental results. |
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Authors:
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Jos? Mario Barichello; Tatsuhiro Ishida; Hiroshi Kiwada |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 605 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 461-72 Citation Subset: IM |
Affiliation:
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Department of Pharmacokinetics and Biopharmaceutics, Subdivision of Biopharmaceutical Sciences, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cations
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chemistry DNA / administration & dosage*, chemistry Gene Silencing Hela Cells Humans Liposomes / chemistry*, classification Plasmids / administration & dosage RNA, Small Interfering / administration & dosage*, chemistry Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Liposomes; 0/RNA, Small Interfering; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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