| Complex porcine model of atherosclerosis: induction of early coronary lesions after long-term hyperlipidemia without sustained hyperglycemia. | |
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MedLine Citation:
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PMID: 19340354 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The incidence of coronary artery disease (CAD) is still increasing in industrialized countries and it is even higher in diabetic patients. For experimental studies investigating the pathophysiology of CAD, the use of an animal model comparable with the pathological situation in patients is crucial. OBJECTIVE: To develop a model of advanced coronary atherosclerosis with induction of hyperlipidemia and hyperglycemia in domestic pigs. METHODS: Six pigs were fed a standard pig chow (controls), two were fed a 2% cholesterol and 17% coconut fat diet (Chol group), and two pigs received a 4% cholesterol and 17% coconut fat diet combined with streptozotocin (STZ) injections to induce diabetes (High Chol+STZ group). Serum lipid and plasma glucose values were analyzed, and histochemical staining for morphometric analysis and immunohistochemistry were performed. RESULTS: Pigs on the hyperlipidemic diet had elevated mean (+/- SD) serum lipid levels (total cholesterol 5.05+/-1.45 mmol/L [Chol] and 5.03+/-2.41 mmol/L [High Chol+STZ] versus 2.09+/-0.23 mmol/L [controls]). Histopathological evaluation revealed an initial stage of coronary atherosclerosis. None of the STZ-treated pigs showed a sustained elevation of plasma glucose (mean glucose before STZ injection was 5.11+/-0.94 mmol/L and thereafter was 6.03+/-2.39 mmol/L) or a decline in pancreatic beta cells. CONCLUSIONS: The current data suggest that the domestic porcine model is not suitable to create severe CAD using an atherogenic diet in combination with STZ injections for experimental interventional vascular research. This may be due to different STZ sensitivities among species. However, hyperlipidemia induced early pathological lesions in coronary arteries resembling initial stages of atherosclerosis without severe luminal narrowing. |
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Authors:
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Sandra Artinger; Carolin Deiner; Christoph Loddenkemper; Peter L Schwimmbeck; Heinz-Peter Schultheiss; Klaus Pels |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Canadian journal of cardiology Volume: 25 ISSN: 1916-7075 ISO Abbreviation: Can J Cardiol Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-02 Completed Date: 2009-04-28 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 8510280 Medline TA: Can J Cardiol Country: Canada |
Other Details:
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Languages: eng Pagination: e109-14 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Charité-Campus Benjamin Franklin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / analysis Coronary Artery Disease* / etiology, pathology Coronary Vessels / metabolism, pathology Diabetes Mellitus, Experimental Diet, Atherogenic Disease Models, Animal* Hyperglycemia / complications Hyperlipidemias / complications Immunohistochemistry Lipids / blood Macrophages / metabolism Male Swine |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Lipids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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