| Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging. | |
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MedLine Citation:
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PMID: 19382913 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Mitochondrial morphology and intracellular organization are tightly controlled by the processes of mitochondrial fission-fusion. Moreover, mitochondrial movement and redistribution provide a local ATP supply at cellular sites of particular demands. Here we analysed mitochondrial dynamics in isolated primary human pancreatic cells. Using real time confocal microscopy and mitochondria-specific fluorescent probes tetramethylrhodamine methyl ester and MitoTracker Green we documented complex and novel patterns of spatial and temporal organization of mitochondria, mitochondrial morphology and motility. The most commonly observed types of mitochondrial dynamics were (i) fast fission and fusion; (ii) small oscillating movements of the mitochondrial network; (iii) larger movements, including filament extension, retraction, fast (0.1-0.3 mum/sec.) and frequent oscillating (back and forth) branching in the mitochondrial network; (iv) as well as combinations of these actions and (v) long-distance intracellular translocation of single spherical mitochondria or separated mitochondrial filaments with velocity up to 0.5 mum/sec. Moreover, we show here for the first time, a formation of unusual mitochondrial shapes like rings, loops, and astonishingly even knots created from one or more mitochondrial filaments. These data demonstrate the presence of extensive heterogeneity in mitochondrial morphology and dynamics in living cells under primary culture conditions. In summary, this study reports new patterns of morphological changes and dynamic motion of mitochondria in human pancreatic cells, suggesting an important role of integrations of mitochondria with other intracellular structures and systems. |
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Authors:
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Andrey V Kuznetsov; Martin Hermann; Jakob Troppmair; Raimund Margreiter; Paul Hengster |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-27 |
Journal Detail:
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Title: Journal of cellular and molecular medicine Volume: 14 ISSN: 1582-4934 ISO Abbreviation: J. Cell. Mol. Med. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-04-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101083777 Medline TA: J Cell Mol Med Country: England |
Other Details:
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Languages: eng Pagination: 417-25 Citation Subset: IM |
Affiliation:
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Daniel Swarovski Research Laboratory, Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University, Innrain, Innsbruck, Austria. andrey.kuznetsov@uki.at |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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