Document Detail


Complex lipid requirements for detergent-solubilized phosphoacetylmuramyl-pentapeptide translocase from Micrococcus luteus.
MedLine Citation:
PMID:  4340168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay. By assay in the presence of lipid, the activity of the Triton-solubilized enzyme could be measured. The synthesis of C(55)-isoprenyl-P-P-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide required C(55)-isoprenyl-P, and was stimulated by a neutral lipid. The exchange reaction of UDP-MurNAc-pentapeptide with UMP required a polar lipid fraction, but the reaction was not affected by C(55)-isoprenyl-P or the neutral lipid. Thus, measurement of activity of the detergent-solubilized enzyme requires addition of three lipids, the lipid substrate (C(55)-isoprenyl-P), the neutral lipid, and a polar lipid.
Authors:
J N Umbreit; J L Strominger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Jul 
Date Detail:
Created Date:  1972-09-22     Completed Date:  1972-09-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1972-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carbon Isotopes
Chloroform
Chromatography, Gel
Enzyme Activation
Glycopeptides
Kinetics
Lipids / pharmacology*
Methanol
Micrococcus / drug effects,  enzymology*
Peptides
Phosphatidylethanolamines / pharmacology
Phosphatidylinositols / pharmacology
Phosphoric Acids
Solubility
Surface-Active Agents / pharmacology
Terpenes
Transferases* / isolation & purification
Uracil Nucleotides
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Glycopeptides; 0/Lipids; 0/Peptides; 0/Phosphatidylethanolamines; 0/Phosphatidylinositols; 0/Phosphoric Acids; 0/Surface-Active Agents; 0/Terpenes; 0/Uracil Nucleotides; 67-56-1/Methanol; 67-66-3/Chloroform; EC 2.-/Transferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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