| Complex lipid requirements for detergent-solubilized phosphoacetylmuramyl-pentapeptide translocase from Micrococcus luteus. | |
| | |
MedLine Citation:
|
PMID: 4340168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay. By assay in the presence of lipid, the activity of the Triton-solubilized enzyme could be measured. The synthesis of C(55)-isoprenyl-P-P-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide required C(55)-isoprenyl-P, and was stimulated by a neutral lipid. The exchange reaction of UDP-MurNAc-pentapeptide with UMP required a polar lipid fraction, but the reaction was not affected by C(55)-isoprenyl-P or the neutral lipid. Thus, measurement of activity of the detergent-solubilized enzyme requires addition of three lipids, the lipid substrate (C(55)-isoprenyl-P), the neutral lipid, and a polar lipid. |
| | |
Authors:
|
J N Umbreit; J L Strominger |
Related Documents
:
|
1417918 - The transfer of phosphatidylserine from rat brain microsomes to mitochondria is regulat... 23429608 - Broadening the catalyst and reaction scope of regio- and chemoselective c-h oxygenation... 16392818 - Influence of various central moieties on the hypolipidemic properties of long hydrocarb... 21782678 - Thiophenes and furans derivatives: a new class of potential pharmacological agents. 10810708 - Unexpected products via singlet oxygen oxygenation of functionalized 5,6-dihydro-1,4-ox... 21495648 - Enantioselective synthesis of β-substituted cyclic ketones via cobalt-catalyzed asymme... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 69 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1972 Jul |
Date Detail:
|
Created Date: 1972-09-22 Completed Date: 1972-09-22 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1972-4 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbon Isotopes Chloroform Chromatography, Gel Enzyme Activation Glycopeptides Kinetics Lipids / pharmacology* Methanol Micrococcus / drug effects, enzymology* Peptides Phosphatidylethanolamines / pharmacology Phosphatidylinositols / pharmacology Phosphoric Acids Solubility Surface-Active Agents / pharmacology Terpenes Transferases* / isolation & purification Uracil Nucleotides |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Isotopes; 0/Glycopeptides; 0/Lipids; 0/Peptides; 0/Phosphatidylethanolamines; 0/Phosphatidylinositols; 0/Phosphoric Acids; 0/Surface-Active Agents; 0/Terpenes; 0/Uracil Nucleotides; 67-56-1/Methanol; 67-66-3/Chloroform; EC 2.-/Transferases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of environmental pH on the efficiency of cellular hybridization.
Next Document: The nature of the positive inotropic response of the isolated frog heart to theophylline and to imin...