Document Detail

Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells.
MedLine Citation:
PMID:  16273259     Owner:  NLM     Status:  MEDLINE    
We have previously reported that significantly higher levels of Keratin 14 (Ker-14) was observed in oral squamous cell carcinoma (OSCC) and severely dysplastic tissues, whereas this expression was reversed in hyperplasia and in mild to moderate dysplasia. In this study, the mechanism of Keratin 14 activation in oral squamous cell carcinoma (OSCC) cell lines (HSC-2, HSC-3 and Ca9-22) was investigated. Reporter analysis demonstrated that an upstream region (-1759/-1629) accounted for efficient promoter activity. Furthermore, electromobility sift and supershift assay demonstrated that interactions of the SP-1/SP-3 complex at the elements resided in -1737/-1702 and -1680/-1652 and may be essential for this activation in OSCC cells.
Shuri Ohkura; Nobuo Kondoh; Akiyuki Hada; Masaaki Arai; Yutaka Yamazaki; Masanobu Sindoh; Masayuki Takahashi; Isamu Matsumoto; Mikio Yamamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncology reports     Volume:  14     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-07     Completed Date:  2006-01-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1577-81     Citation Subset:  IM    
Department of Biochemistry II, National Defense Medical College, 3-2 Namiki, Tokorozawa-shi, Saitama 359-8513, Japan.
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MeSH Terms
Binding Sites / genetics
Carcinoma, Squamous Cell / genetics,  pathology
Cell Line, Tumor
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Neoplastic
Keratins / genetics*
Luciferases / genetics,  metabolism
Molecular Sequence Data
Mouth Neoplasms / genetics,  pathology
Oligonucleotide Probes / genetics,  metabolism
Promoter Regions, Genetic / genetics
Protein Binding
RNA, Messenger / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Regulatory Sequences, Nucleic Acid / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Sp1 Transcription Factor / metabolism*
Sp3 Transcription Factor / metabolism*
Transcription, Genetic
Reg. No./Substance:
0/KRT14 protein, human; 0/Keratin-14; 0/Oligonucleotide Probes; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Sp1 Transcription Factor; 148710-94-5/Sp3 Transcription Factor; 68238-35-7/Keratins; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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