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Complex effects of imatinib on spontaneous and oxytocin-induced contractions in human non-pregnant myometrium.
MedLine Citation:
PMID:  21893472     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Human myometrium includes two important cell populations involved in its contractility: smooth muscle fibers and interstitial cells. The pacemaking mechanism is not yet identified, but it is possible that myometrial smooth muscle cells contract in response to a signal generated by c-kit positive interstitial cells. The aim of this study was to investigate the effects of imatinib as a c-kit receptor antagonist on the spontaneous or oxytocin (OT) induced contractions of human non-pregnant myometrium in vitro. Myometrial strips were obtained from non-pregnant women (reproductive age) undergoing hysterectomy for benign indications. The strips were suspended in organ baths for recording of isometric tension. Imatinib effects were assessed on spontaneous contraction and after preexposure to OT.Direct exposure of myometrial strips to imatinib inhibits both amplitude and frequency of contractions (80-320 μM) in a dose dependent manner. Amplitude reverted back to 90% of the baseline amplitude by consequent addition of imatinib (until 480 μM). Total inhibition of myometrial contraction was obtained after addition of OT 60 nM. If myometrium was pre-exposed to OT (320 nM), imatinib 80-160 μm increased amplitude, while decreasing frequency. These data provide evidence that telocytes may be involved as modulators of the spontaneous contractions of the non-pregnant human uterus, via a tyrosine-kinase independent signaling pathway.
Authors:
S M Cretoiu; A A Simionescu; L Caravia; A Curici; D Cretoiu; L M Popescu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta physiologica Hungarica     Volume:  98     ISSN:  0231-424X     ISO Abbreviation:  Acta Physiol Hung     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309201     Medline TA:  Acta Physiol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  329-38     Citation Subset:  IM    
Affiliation:
'Carol Davila' University of Medicine and Pharmacy Department of Cellular and Molecular Medicine Bucharest Romania Filantropia Hospital 'V. Babeş' National Institute of Pathology, Bucharest, and Obstetrics and Gynecology Bucharest Romania.
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