Document Detail


Complete solubilization of formalin-fixed, paraffin-embedded tissue may improve proteomic studies.
MedLine Citation:
PMID:  23339100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue-based proteomic approaches (tissue proteomics) are essential for discovering and evaluating biomarkers for personalized medicine. In any proteomics study, the most critical issue is sample extraction and preparation. This problem is especially difficult when recovering proteins from formalin-fixed, paraffin-embedded (FFPE) tissue sections. However, improving and standardizing protein extraction from FFPE tissue is a critical need because of the millions of archival FFPE tissues available in tissue banks worldwide. Recent progress in the application of heat-induced antigen retrieval principles for protein extraction from FFPE tissue has resulted in a number of published FFPE tissue proteomics studies. However, there is currently no consensus on the optimal protocol for protein extraction from FFPE tissue or accepted standards for quantitative evaluation of the extracts. Standardization is critical to ensure the accurate evaluation of FFPE protein extracts by proteomic methods such as reverse phase protein arrays, which is now in clinical use. In our view, complete solubilization of FFPE tissue samples is the best way to achieve the goal of standardizing the recovery of proteins from FFPE tissues. However, further studies are recommended to develop standardized protein extraction methods to ensure quantitative and qualitative reproducibility in the recovery of proteins from FFPE tissues.
Authors:
Shan-Rong Shi; Clive R Taylor; Carol B Fowler; Jeffrey T Mason
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2013-03-06
Journal Detail:
Title:  Proteomics. Clinical applications     Volume:  7     ISSN:  1862-8354     ISO Abbreviation:  Proteomics Clin Appl     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-15     Completed Date:  2013-12-18     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  101298608     Medline TA:  Proteomics Clin Appl     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  264-72     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / analysis
Fixatives / chemistry*
Formaldehyde / chemistry*
Humans
Paraffin / chemistry*
Paraffin Embedding / methods*
Proteome / analysis*
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
1 R42CA122715/CA/NCI NIH HHS; 1R21CA134359/CA/NCI NIH HHS; R42 CA122715/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fixatives; 0/Proteome; 1HG84L3525/Formaldehyde; 8002-74-2/Paraffin
Comments/Corrections

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