Document Detail

Complete nucleotide sequence and tissue-specific expression of the rat phenylethanolamine N-methyltransferase gene.
MedLine Citation:
PMID:  7931317     Owner:  NLM     Status:  MEDLINE    
The rat phenylethanolamine N-methyltransferase (PNMT) gene was isolated from a genomic library by cross-hybridization with a bovine PNMT cDNA probe. Complete nucleotide sequence analysis of a genomic clone showed that this gene contained three exons and spanned about 2.8 kb in length. There were the acute-phase response element, TATA, SP1, and GRE sequences. The physicochemical properties of rat adrenal PNMT were different from those of the brainstem PNMT. However, northern blot and reverse transcription-polymerase chain reaction analysis showed that the rat PNMT gene may not express the multiple forms of mRNA. These results suggest that the rat PNMT gene might produce a single enzyme protein, whose activity may be differentially modulated by tissue-specific environment in the central and peripheral systems.
Y H Suh; Y S Chun; I S Lee; S S Kim; W Choi; Y H Chong; L Hong; S H Kim; C W Park; C G Kim
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  63     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1994-11-16     Completed Date:  1994-11-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1603-8     Citation Subset:  IM    
Department of Pharmacology, College of Medicine, Seoul National University, South Korea.
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MeSH Terms
Adrenal Glands / chemistry,  enzymology
Amino Acid Sequence
Base Sequence
Blotting, Northern
Brain Stem / chemistry,  enzymology
DNA / analysis,  genetics*
Gene Expression Regulation, Enzymologic
Molecular Sequence Data
Organ Specificity
Phenylethanolamine N-Methyltransferase / analysis,  genetics*
Polymerase Chain Reaction
RNA, Messenger / analysis,  genetics
Sequence Analysis, DNA*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Reg. No./Substance:
0/RNA, Messenger; 9007-49-2/DNA; EC N-Methyltransferase

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