| Complete nucleotide sequence and tissue-specific expression of the rat phenylethanolamine N-methyltransferase gene. | |
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MedLine Citation:
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PMID: 7931317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The rat phenylethanolamine N-methyltransferase (PNMT) gene was isolated from a genomic library by cross-hybridization with a bovine PNMT cDNA probe. Complete nucleotide sequence analysis of a genomic clone showed that this gene contained three exons and spanned about 2.8 kb in length. There were the acute-phase response element, TATA, SP1, and GRE sequences. The physicochemical properties of rat adrenal PNMT were different from those of the brainstem PNMT. However, northern blot and reverse transcription-polymerase chain reaction analysis showed that the rat PNMT gene may not express the multiple forms of mRNA. These results suggest that the rat PNMT gene might produce a single enzyme protein, whose activity may be differentially modulated by tissue-specific environment in the central and peripheral systems. |
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Authors:
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Y H Suh; Y S Chun; I S Lee; S S Kim; W Choi; Y H Chong; L Hong; S H Kim; C W Park; C G Kim |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurochemistry Volume: 63 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1994-11-16 Completed Date: 1994-11-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1603-8 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, College of Medicine, Seoul National University, South Korea. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/X75333 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Glands
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chemistry,
enzymology Amino Acid Sequence Animals Base Sequence Blotting, Northern Brain Stem / chemistry, enzymology Cattle DNA / analysis, genetics* Gene Expression Regulation, Enzymologic Humans Mice Molecular Sequence Data Organ Specificity Phenylethanolamine N-Methyltransferase / analysis, genetics* Polymerase Chain Reaction RNA, Messenger / analysis, genetics Rats Sequence Analysis, DNA* Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9007-49-2/DNA; EC 2.1.1.28/Phenylethanolamine N-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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