Document Detail

Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus.
MedLine Citation:
PMID:  18514348     Owner:  NLM     Status:  MEDLINE    
The complete nucleotide sequence of polyprotein gene 1 and the assembled full-length genome sequence are presented for turkey coronavirus (TCoV) isolates 540 and ATCC. The TCoV polyprotein gene encoded two open reading frames (ORFs), which are translated into two products, pp1a and pp1ab, the latter being produced via -1 frameshift translation. TCoV polyprotein pp1a and pp1ab were predicted to be processed to 15 non-structure proteins (nsp2-nsp16), with nsp1 missing. ClustalW analysis revealed 88.99% identity and 96.99% similarity for pp1ab between TCoV and avian infectious bronchitis virus (IBV) at the amino acid level. The whole genome consists of 27,749 nucleotides for 540 and 27,816 nucleotides for ATCC, excluding the poly(A) tail. A total of 13 ORFs were predicted for TCoV. Five subgenomic RNAs were detected from ATCC-infected turkey small intestines by Northern blotting. The whole genome sequence had 86.9% identity between TCoV and IBV, supporting that TCoV is a group 3 coronavirus.
Jianzhong Cao; Ching-Ching Wu; Tsang Long Lin
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Publication Detail:
Type:  Journal Article     Date:  2008-06-02
Journal Detail:
Title:  Virus research     Volume:  136     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-07-08     Completed Date:  2008-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  43-9     Citation Subset:  IM    
Department of Comparative Pathobiology, 406 South University Street, Purdue University, West Lafayette, IN 47907, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU022525;  EU022526
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MeSH Terms
Base Sequence
Coronavirus, Turkey / genetics*
Frameshifting, Ribosomal
Gene Order*
Genome, Viral*
Infectious bronchitis virus / genetics
Molecular Sequence Data
Open Reading Frames
Polyproteins / genetics*
RNA, Viral / genetics,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Viral Proteins / genetics*
Reg. No./Substance:
0/Polyproteins; 0/RNA, Viral; 0/Viral Proteins

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