| Complete inhibition of creatine kinase in isolated perfused rat hearts. | |
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MedLine Citation:
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PMID: 2949626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transient exposure of an isolated isovolumic perfused rat heart to low concentrations (0.5 mM) of perfusate-born iodoacetamide resulted in complete inhibition of creatine kinase and partial inhibition of glyceraldehyde-3-phosphate dehydrogenase in the heart. At low levels of developed pressure, hearts maintained mechanical function, ATP, and creatine phosphate levels at control values. However, iodoacetamide-inhibited hearts were unable to maintain control values of end diastolic pressure or peak systolic pressure as work load increased. Global ischemia resulted in loss of all ATP without loss of creatine phosphate, indicating lack of active creatine kinase. These results indicate that isovolumic perfused rat hearts are able to maintain normal function and normal levels of high-energy phosphates without active creatine kinase at low levels of developed pressure. |
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Authors:
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E T Fossel; H Hoefeler |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: The American journal of physiology Volume: 252 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1987 Jan |
Date Detail:
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Created Date: 1987-03-02 Completed Date: 1987-03-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E124-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Creatine Kinase / antagonists & inhibitors* Glyceraldehyde-3-Phosphate Dehydrogenases / antagonists & inhibitors Hexokinase / metabolism Iodoacetamide / pharmacology Kinetics Magnetic Resonance Spectroscopy / methods Male Myocardium / enzymology* Perfusion Phosphofructokinase-1 / metabolism Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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144-48-9/Iodoacetamide; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases; EC 2.7.1.1/Hexokinase; EC 2.7.1.11/Phosphofructokinase-1; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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