Document Detail

Complete inhibition of creatine kinase in isolated perfused rat hearts.
MedLine Citation:
PMID:  2949626     Owner:  NLM     Status:  MEDLINE    
Transient exposure of an isolated isovolumic perfused rat heart to low concentrations (0.5 mM) of perfusate-born iodoacetamide resulted in complete inhibition of creatine kinase and partial inhibition of glyceraldehyde-3-phosphate dehydrogenase in the heart. At low levels of developed pressure, hearts maintained mechanical function, ATP, and creatine phosphate levels at control values. However, iodoacetamide-inhibited hearts were unable to maintain control values of end diastolic pressure or peak systolic pressure as work load increased. Global ischemia resulted in loss of all ATP without loss of creatine phosphate, indicating lack of active creatine kinase. These results indicate that isovolumic perfused rat hearts are able to maintain normal function and normal levels of high-energy phosphates without active creatine kinase at low levels of developed pressure.
E T Fossel; H Hoefeler
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  252     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-03-02     Completed Date:  1987-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E124-9     Citation Subset:  IM    
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MeSH Terms
Creatine Kinase / antagonists & inhibitors*
Glyceraldehyde-3-Phosphate Dehydrogenases / antagonists & inhibitors
Hexokinase / metabolism
Iodoacetamide / pharmacology
Magnetic Resonance Spectroscopy / methods
Myocardium / enzymology*
Phosphofructokinase-1 / metabolism
Rats, Inbred Strains
Reg. No./Substance:
144-48-9/Iodoacetamide; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases; EC; EC; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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