Document Detail


Complete globozoospermia associated with PLCζ deficiency treated with calcium ionophore and ICSI results in pregnancy.
MedLine Citation:
PMID:  20133201     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Globozoospermia is an infrequent pathology in which spermatozoa lack acrosomes. Patients are considered sterile without IVF augmented with intracytoplasmic sperm injection (ICSI), as fertilization is impaired due to absence of oocyte activation. As far as is known, this is the first study to report results of a comprehensive approach to the treatment of the semen parameters, sperm DNA fragmentation, aneuploidy, transmission electron microscopy, Western blotting and immunofluorescence for detection of phospholipase C zeta (PLCzeta), as well as ICSI outcome, of an affected patient. Morphological evaluation and transmission electron microscopy revealed complete globozoospermia with significant duplicate heads and tails. Analysis for DNA damage revealed fragmentation rates of approximately 80% in semen and 15-23% in swim-up fractions. PLCzeta was not detected by immunofluorescence or Western blotting. Aneuploidy rates were within normal ranges. ICSI followed by oocyte activation with calcium ionophore resulted in high rates of fertilization, and an ongoing pregnancy was established after transfer of cryopreserved-thawed embryos.
Authors:
S L Taylor; S Y Yoon; M S Morshedi; D R Lacey; T Jellerette; R A Fissore; S Oehninger
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-12-28
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  20     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-30     Completed Date:  2010-12-20     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  559-64     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Acrosome / pathology
Adult
Calcium / metabolism
DNA Fragmentation
Embryo Transfer
Female
Humans
Infertility, Male / therapy
Ionophores / therapeutic use
Male
Phosphoinositide Phospholipase C / deficiency*
Pregnancy
Semen Analysis
Sperm Injections, Intracytoplasmic
Spermatozoa / abnormalities*
Treatment Outcome
Grant Support
ID/Acronym/Agency:
HD051872/HD/NICHD NIH HHS; R01 HD051872/HD/NICHD NIH HHS; R01 HD051872-03/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Ionophores; EC 3.1.4.11/PLCZ1 protein, human; EC 3.1.4.11/Phosphoinositide Phospholipase C; SY7Q814VUP/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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