| Complete globozoospermia associated with PLCζ deficiency treated with calcium ionophore and ICSI results in pregnancy. | |
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MedLine Citation:
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PMID: 20133201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Globozoospermia is an infrequent pathology in which spermatozoa lack acrosomes. Patients are considered sterile without IVF augmented with intracytoplasmic sperm injection (ICSI), as fertilization is impaired due to absence of oocyte activation. As far as is known, this is the first study to report results of a comprehensive approach to the treatment of the semen parameters, sperm DNA fragmentation, aneuploidy, transmission electron microscopy, Western blotting and immunofluorescence for detection of phospholipase C zeta (PLCzeta), as well as ICSI outcome, of an affected patient. Morphological evaluation and transmission electron microscopy revealed complete globozoospermia with significant duplicate heads and tails. Analysis for DNA damage revealed fragmentation rates of approximately 80% in semen and 15-23% in swim-up fractions. PLCzeta was not detected by immunofluorescence or Western blotting. Aneuploidy rates were within normal ranges. ICSI followed by oocyte activation with calcium ionophore resulted in high rates of fertilization, and an ongoing pregnancy was established after transfer of cryopreserved-thawed embryos. |
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Authors:
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S L Taylor; S Y Yoon; M S Morshedi; D R Lacey; T Jellerette; R A Fissore; S Oehninger |
Publication Detail:
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Type: Case Reports; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-12-28 |
Journal Detail:
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Title: Reproductive biomedicine online Volume: 20 ISSN: 1472-6491 ISO Abbreviation: Reprod. Biomed. Online Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-30 Completed Date: 2010-12-20 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101122473 Medline TA: Reprod Biomed Online Country: England |
Other Details:
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Languages: eng Pagination: 559-64 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome
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pathology Adult Calcium / metabolism DNA Fragmentation Embryo Transfer Female Humans Infertility, Male / therapy Ionophores / therapeutic use Male Phosphoinositide Phospholipase C / deficiency* Pregnancy Semen Analysis Sperm Injections, Intracytoplasmic Spermatozoa / abnormalities* Treatment Outcome |
| Grant Support | |
ID/Acronym/Agency:
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HD051872/HD/NICHD NIH HHS; R01 HD051872-03/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ionophores; 7440-70-2/Calcium; EC 3.1.4.11/PLCZ1 protein, human; EC 3.1.4.11/Phosphoinositide Phospholipase C |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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