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Complete Monosaccharide Analysis by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection.
MedLine Citation:
PMID:  22448871     Owner:  NLM     Status:  Publisher    
Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high performance anion-exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method is to profile a complete set of monosaccharides, and has a limit of quantitation of ~12.5 pmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and N-glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed.
Zhenqing Zhang; Nazeer M Khan; Karen M Nunez; Edward K Chess; Christina M Szabo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-24
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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