| Complete Monosaccharide Analysis by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection. | |
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MedLine Citation:
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PMID: 22448871 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high performance anion-exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method is to profile a complete set of monosaccharides, and has a limit of quantitation of ~12.5 pmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and N-glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed. |
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Authors:
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Zhenqing Zhang; Nazeer M Khan; Karen M Nunez; Edward K Chess; Christina M Szabo |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-24 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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