Document Detail


Complementation studies with Rous sarcoma virus gag and gag-pol polyprotein mutants.
MedLine Citation:
PMID:  1316486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Avian retroviruses (with the notable exception of spleen necrosis virus) express their protease (PR) both in their gag and their gag-pol polyprotein precursors, in contrast to other retroviruses, notably, the mammalian retroviruses, in which PR is encoded in the gag-pol polyprotein or in a separate reading frame as a gag-pro product. The consequence is that the avian PR is expressed in stoichiometric rather than catalytic amounts. To investigate the significance of the particular genome organization of the avian retrovirus prototype Rous sarcoma virus, we developed an assay that measures complementation between the gag and the gag-pol polyproteins by expressing them from two different plasmids in transfected cells. By using this assay, we showed that the protease PR from the gag-pol polyprotein is capable of autocatalytic self-cleavage and -activation when coexpressed with a protease-deficient gag protein and that the PR domain has a role in viral particle assembly. Furthermore, this complementation assay can be used to investigate the role of the gag domain in the gag-pol polyprotein by determining whether it can rescue a defect in the gag polyprotein. We report here the results of such an experiment, which studied a mutation in the N terminus of the gag gene.
Authors:
S Oertle; N Bowles; P F Spahr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-06-16     Completed Date:  1992-06-16     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3873-8     Citation Subset:  IM; X    
Affiliation:
Department of Molecular Biology, University of Geneva, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Avian Sarcoma Viruses / genetics*
Base Sequence
Chick Embryo
DNA Mutational Analysis
Endopeptidases / genetics*,  metabolism
Enzyme Activation
Fusion Proteins, gag-pol / biosynthesis,  genetics*
Gene Products, gag / genetics
Gene Products, pol / genetics
Genetic Complementation Test
Molecular Sequence Data
Protein Processing, Post-Translational
Retroviridae Proteins / genetics*,  metabolism
Virion / growth & development
Chemical
Reg. No./Substance:
0/Fusion Proteins, gag-pol; 0/Gene Products, gag; 0/Gene Products, pol; 0/Retroviridae Proteins; 0/avian retrovirus proteins; EC 3.4.-/Endopeptidases
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